Pregled bibliografske jedinice broj: 1225238
Mechanisms of macrolide resistance in clinical isolates of Haemophilus influenzae with higher macrolide MICs than baseline strains from Alexander Project (1997-2000)
Mechanisms of macrolide resistance in clinical isolates of Haemophilus influenzae with higher macrolide MICs than baseline strains from Alexander Project (1997-2000) // 42nd ICAAC Abstracts, ASM
San Diego (CA), Sjedinjene Američke Države: ASM, 2002. str. 76-76 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1225238 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Mechanisms of macrolide resistance in clinical
isolates of Haemophilus influenzae with higher
macrolide MICs than baseline strains from
Alexander Project (1997-2000)
Autori
Peric, Mihaela ; Bozdogan, Bulent ; Jacobs, Michael R. ; Appelbaum, Peter C.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
42nd ICAAC Abstracts, ASM
/ - : ASM, 2002, 76-76
Skup
42nd ICAAC
Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 27.09.2002. - 30.09.2002
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
macrolide, resistance. H. influenzae
Sažetak
Background: Macrolide hyper-susceptible strains were detected among clinical isolates collected for the Alexander Project. The purpose of this study was to determine the mechanism of their hyper-susceptibility. Materials and methods: Macrolide hyper-susceptible strains were defined as isolates with Azi MICs of <0.25 g/ml or Cla MICs of <2 g/ml. Macrolide hyper-susceptible, baseline (0.25-4 g/ml for Azi, 2-16 g/ml for Cla), and resistant (>4 g/ml for Azi, >16 g/ml for Cla) strains were studied for radioactive Azi and Cla accumulation, in presence or absence of CCCP, an efflux inhibitor. Results: A total of 28 hyper-susceptible , 20 baseline, and 22 resistant strains were studied. Radioactive Azi and Cla accumulation was higher among hyper-susceptible strains. Treatment with CCCP increased radioactive azithromycin and clarithromycin accumulation in baseline strains and macrolide resistant strains, which shows the presence of efflux mechanism in these strains. However for hyper-susceptible strains the effect of CCCP on the accumulation of macrolides was at least 3 fold lower than baseline and resistant strains, which indicates absence of efflux mechanism in these strains. Conclusion: Azithromycin and clarithromycin hyper- susceptibility was found to be associated with the absence of efflux mechanism. Efflux mechanism was present among baseline and macrolide resistant clinical H. influenzae strains.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
