Naslov
Canine Adipose Derived Mesenchymal Stem Cells Support Canid Alphaherpesvirus 1 Infection In Vitro

Autori
Nina Krešić, Marina Prišlin, Ivana Ljolje, Šimun Naletilić, Petar Kostešić, Dunja Vlahović, Željko Mihaljević, Dragan Brnić, Nenad Turk, Boris Habrun

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ESVV2022 Abstract Book / - Ghent : ESVV, 2022, 157-157

Skup
12th International Congress for Veterinary Virology (ESVV 2022)

Mjesto i datum
Gent, Belgija, 20.09.2022. - 23.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
canine, herpesvirus infection, permissiveness, stem cells

Sažetak
Since the 1960s Canid alphaherpesvirus 1, a Varicellovirus has been recognized to cause fatal hemorrhagic disease in puppies, ocular, respiratory and reproductive disorders in adult canines. Massive infection of fetal tissues, cell- associated viremia and replication in vascular endothelium are highlights of CHV-1 infection in vivo. Mesenchymal stem cells are located in perivascular area, tether, circulate and home to injured tissues and it is unknown if they could present CHV-1 targets. We sought their permissiveness and ability to support CHV-1 infection in vitro in comparison to MDCK, a CHV-1 primary choice for infection in vitro. Furthemore, infection-induced changes in gene expression using array technology were analysed. To deliver the aims, canine mesenchymal stem cell cultures from abdominal adipose tissue (cASCs) of ten females were established in line with ISCT requirements. Initiated cultures were exposed in two passages to CHV-1 field strain, isolated from 6-day-old golden retriever organs. Spindle-shaped morphology, a lifetime up to six passages in culture, preserved trilineage differentiation and immunophenotype characterized cASCs cultivation. The first signs of CHV-1 CPE in cASCs ocurring at 30h p.i. in the form of cell rounding, further progressing to clustering already rounded cells and developing characteristic CPE to the full extent in 72-96h were hallmarks of CHV-1 infection in vitro. CHV-1 achieved stable titers in MDCK at MOI 0, 1, 0, 5 and 1 while CHV-1 titers in cAD-MSCS were variable between donors at different MOI and on average lower than in MDCK. CHV-1 significantly affected expression in 20, 23% of genes with overexpression of genes involved in cell growth, chondrocyte differentiation, encoding signaling proteins, maintenance of cell shape and cytoplasm integrity. Downregulated genes regulate cell survival, proliferation, are a component of class I major histocompatibility complex involved in the presentation of peptide antigens to the immune system and membrane receptors involved in cell adhesion.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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