Naslov
Optimization of environmentally friendly Plantago major L ultrasound-assisted extraction

Autori
Marijan, Marijan ; Jakupović, Lejsa ; Zovko Končić , Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
70th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA 2022)

Mjesto i datum
Solun, Grčka, 28.08.2022. - 31.08.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Plantago major ; Optimization ; Phenolics ; Aucubin ; Verbascoside

Sažetak
Plantago major L. is a species of flowering plant from the plantain family Plantaginaceae native to most of Europe and northern and central Asia, but widely naturalised worldwide [1]. In this work, green solvent-based ultrasound-assisted extraction of P. major bioactive phenolics was investigated in two steps. In the first step, 2-level factorial design was utilized for singling out the factors that impact the extraction efficiency of total phenols (TP), total phenolic acid (TPA), aucubin (Auc), and verbascoside (Ver), as dependant variables. Their content was determined by HPLC and spectrophotometric methods. The impact of the following independent variables was analyzed: glycerol content, 2-hydroxypropyl-β-cyclodextrin concentration, temperature, extraction duration, ultrasound power, and lactic acid content. The utilized 2-level factorial design indicated that glycerol content, temperature, ultrasound power, and lactic acid content significantly impacted extraction efficiency. In the second step, Box Behnken design was applied for the fine-tuning of the significant independent variables required to attain the maximum quantity of the target compounds. Four extracts, each containing the maximum of one target response, were prepared and the content of the target compounds determined. The prepared extracts were rich in phenolic compounds. The observed values for the responses were in good accordance with the predicted ones, the deviation being lower than 5% (Table 1). The results have shown that the ultrasound-assisted extraction of P. major phenolics is an acceptable green alternative to traditional organic solvent-based extraction.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA