Naslov
Viruses of Sour Cherry cv. Maraska in plantation ‚Vlačine‛, Croatia

Autori
Ražov, Josip ; Šimon, Silvio ; Vončina, Darko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
GEA (Geo Eco-Eco Agro) International Conference - Book of Abstracts / Spalević, Velibor - Podgorica : Faculty of Architecture - University of Montenegro, Faculty of Philosophy - University of Montenegro, Biotecnical faculty - University of Montenegro, 2020, 71-71

ISBN
978-86-86625-27-4

Skup
GEA (Geo Eco-Eco Agro) International Conference

Mjesto i datum
Podgorica, Crna Gora, 28.05.2020. - 31.05.2020

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Recenziran

Ključne riječi
viruses ; sour cherry ; ELISA ; RT-PCR, sanitary selection

Sažetak
Sour cherry var. Maraska (Prunus cerasus var. Marasca) is considered Croatian autochthonous variety dominantly produced in the region of Ravni kotari (North Dalmatia). Its origin dates to 14th century and is mainly used for liquor production (Cherry brandy, Maraschino) with big importance for local agriculture. In addition to its commercial value data about virus occurrence and frequency date back to 1980’s. Two hundred trees from plantation Vlačine (200 ha) were selected and screened by enzyme-linked immunosorbent assay (ELISA) on the presence of 11 viruses: Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Petunia asteroid mosaic virus (PAMV), Plum pox virus (PPV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV) and Tomato black ring virus (TBRV). Additionally, 17 ELISA-virus free trees were tested by reverse transcription polymerase chain reaction (RT-PCR) on four viruses: Cherry mottle leaf virus (ChMLV), Cherry green ring mottle virus (CGRMV) and Little cherry virus 1 and 2 (LChV-1 and LChV-2). Samples of leaves were collected before harvest (June), while shoots were collected during dormancy (November) and used as a source of antigen. ELISA- results confirmed the presence of eight viruses: PNRSV (99 trees, 48, 3%), PAMV and PDV (10 trees, 4.9%), RpRSV (7 trees, 3.4%), CLRV, ArMV and PPV (3 trees, 1.5%) and ApMV (1 tree, 0.5%). In six trees presence of LChV-2 was confirmed by RT-PCR. Symptoms observed in the field were necrotic ringspots on leaves (PNRV), yellowing and reduced growth (PDV), shoot tip deformations and bark cracking (PeAMV), leaf mosaic (PPV, ApMV), uneven fruit ripening (LChV-2). Significant number of infected trees were asymptomatic. Finally, 11 trees free of all 15 tested viruses were selected as potential mother plants for further pomological investigation and production of virus-free planting material.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA