Naslov
Occurrence and Distribution Patterns of Plum Tree Viruses and Genetic Diversity of Sharka Isolates in Bosnia and Herzegovina

Autori
Okić, Arnela ; Wetzel, Thierry ; Nourinejhad Zarghani, Shaheen ; Massart, Sébastien ; Grahić, Jasmin ; Gaši, Fuad ; Konjić, Almira ; Vončina, Darko

Izvornik
Horticulturae (2311-7524) 8 (2022), 9; 8090783, 15

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
plant viruses ; virus survey ; symptom evaluation ; genetic diversity ; plum pox virus ; apple mosaic virus ; prune dwarf virus ; prunus necrotic ringspot virus ; plum bark necrosis stem pitting-associated virus ; apple chlorotic leafspot virus ; myrobalan latent ringspot virus

Sažetak
In order to fill in a decade-long information gap regarding the biological, serological and molecular data for plum tree viruses in Bosnia and Herzegovina, a three-phase study combining symptom evaluation, and serological and molecular assays with high-throughput sequencing (HTS) technology was conducted. The most frequently observed symptoms were discolorations in the form of ring patterns, bands and irregular shapes, as well as vein banding. Sharka-associated symptoms in the form of ring patterns and semicircles were prevalent. A total of 468 plum tree samples were tested by ELISA for the presence of PPV, ApMV, PDV, PNRSV, PBNSPaV, ACLSV and MLRSV. An overall infection incidence of 51.9% was detected, with PPV being the most prevalent (48.7%), followed by PDV (2.99%), PNRSV (0.21%) and mixed infections of PPV+PDV (1.71%). RT-PCR-assisted strain typing in 45 samples revealed PPV-D as the most common strain (22.22%), followed by PPV-REC (6.66%). Mixed infections of PPV-D+PPV-REC were detected (6.66%). HTS enabled the recovery of a 9743 nts long sequence of PPV-D (PPV_O7/80, MW412433), which shared the highest nucleotide and amino acid identities with isolates S13 (LC375131) from Serbia, SVN1 (LC375132) from Slovenia and N9 (LC375129) from Bulgaria. The phylogenetic analysis of the whole genome placed the isolate of the D strain in a distinctive group with the Slovenian isolate SVN1 (LC375132). In addition, the (Cter)NIb/(Nter)CP fragment of a PPV-REC isolate (MW412434) obtained in this survey formed a separate group with previously known isolates from Bosnia and Herzegovina (BOS64Pl and BOS257Pl).

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Interdisciplinarne biotehničke znanosti

POVEZANOST RADA