Naslov
CRKL, AIFM3 and UBASH3A expression during human kidney development
(CRKL, AIFM3 and UBASH3A expression duringhuman kidney development)

Autori
Mirela Lozić, Katarina Vukojević

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
FEBS Open Bio / - , 2022, 328-328

Skup
The Biochemistry Global Summit

Mjesto i datum
Lisabon, Portugal, 06.07.2022. - 14.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CRKL, UBASH3A, AIFM3

Sažetak
We aimed to investigate the spatio-temporal expression of possible CAKUT candidate genes CRKL, AIFM3, and UBASH3A, as well as AIF, CRK, and BCL2 during human kidney development. Human fetal kidney tissue was stained with antibodies and analyzed by fluorescence microscopy and RT-PCR. Quantification of positive cells was assessed by calculation of area percent age and counting cells in nephron structures. ImageJ software was utilized for cell quantitative evaluation of immunoreactivity. Figures were prepared for analysis using subtraction of the median filter and color thresholding to measure the section percentage area covered by the positive signal. Co-localization ofAIFM3 and AIF was calculated by dividing the area of overlap with the combined area of the two using Adobe Photoshop. Results showed statistically significant differences in the temporal expression patterns of the examined markers, depending on investigated developmental stage. Limited but strong expression of CRKL was seen in developing kidneys, with increasing expression up to the period where the majority of nephrons are formed. Results also lead us to conclude that AIFM3 and AIF are important for promoting cell survival, but only AIFM3 is considered a CAKUT candidate gene due to the lack of AIF in nephron developmental structures. Our findings imply the great importance of AIFM3 in energy production in nephrogenesis and tubular maturation. UBASH3A raw scores showed greater immunoreactivity in developing structures than mature one which would point to a meaningful role in nephrogenesis. Also, its spatial relationship with CRK (an important paralog ofCRKL) was examined due to a possible interaction between them but yielded minimal results. The fact that mRNA and proteins ofCRKL, UBASH3A and AIFM3 were detected in all phases of kidney development implies their role as renal development control genes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA