Naslov
Gene expression and cytokine secretion profiles of primary monocytes in response to orthohantavirus infection

Autori
Svoboda, Petra ; Cvetko Krajinović, Lidija ; Bosnar, Martina ; Eraković Haber, Vesna ; Kurolt, Ivan-Christian ; Markotić, Alemka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of the Croatian Immunological Society 2021 Abstracts book / - , 2021, 64-64

Skup
Annual meeting of the Croatian Immunological Society 2021

Mjesto i datum
Trogir, Hrvatska, 23.09.2021. - 25.09.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Puumala, Tula, in vitro

Sažetak
We aimed to investigate gene expression and cytokine secretion of primary monocytes in response to orthohantavirus infection in a timely manner. For this purpose, we analyzed the gene expression dynamics of selected immune and lineage genes, as well as the secretion patterns of selected cytokines and chemokines. Primary monocytes were isolated from six healthy donors and infected with pathogenic (PUUV) or low pathogenic (TULV) orthohantavirus. Monocytes infected with PUUV, TULV or mock control (MOCK) were cultured for up to seven days post infection and at the indicated time points the cells were lysed. qPCR array for 19 genes was performed. The gene expressions were normalised to MOCK and compared between PUUV, TULV and MOCK infection. Concentration dynamics of 21 cytokines and chemokines in supernatants of PUUV-, TULV- or MOCK infected cells, at indicated time points, were measured using magnetic bead-based immunoassays with reads on Luminex 200 analyzer. Orthohantavirus infection triggers early proinflammatory response of primary human monocytes. Prolonged infection further induces differentiation of primary human monocytes into macrophages, shaping the “M2-like“ polarization profile. Significant differences between viruses regarding their pathogenicity were seen at both gene and cytokine and chemokine release levels.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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