Pregled bibliografske jedinice broj: 122213
DNA extraction from archival Giemsa-stained bone-marrow slides: comparison of six rapid methods.
DNA extraction from archival Giemsa-stained bone-marrow slides: comparison of six rapid methods. // British journal of haematology, 101 (1998), 2; 349-351 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 122213 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
DNA extraction from archival Giemsa-stained bone-marrow slides: comparison of six rapid methods.
Autori
Vince, A. ; Poljak, M. ; Seme, K.
Izvornik
British journal of haematology (0007-1048) 101
(1998), 2;
349-351
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Sažetak
The ability of six rapid DNA extraction procedures to provide DNA for the polymerase chain reaction from archival Giemsa-stained bone marrow slides was tested on 120 samples. Boiling in distilled water, freeze-thaw method, boiling in 10% Chelex-100 resin solution, proteinase K/Tween 20/NP-40 method coupled with simplified phenol/ chloroform/isoamyl alcohol protocol or salting-out procedure using saturated NaCl and modification of commercial QIAamp procedure (Qiagen. Chatsworth, Calif.) gave DNA extraction efficiencies of 50%, 70%, 85%, 95%, 100% and 100%, respectively. Our results demonstrate that rough DNA extraction methods have decreased efficiencies compared to complete DNA extraction protocols and that the latter are required to ensure highly reproducible results from archival Giemsa-stained bone marrow slides.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- Index Medicus
