Naslov
EXPRESSION PATTERN OF APOPTOTIC INDUCING FACTOR IN THE INNER EAR DEVELOPMENT OF YOTARI (DAB1 -/-) AND WILD TYPE MICE

Autori
Azra Kazazić, Nela Kelam, Anita Racetin, Natalija Filipović, Yu Katsuyama4, Katarina Vukojević

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Bioanthropology / - Zagreb : Institut za antropologiju, 2022, 172-172

Skup
12th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individidualized Medicine

Mjesto i datum
Dubrovnik, Hrvatska, 22.06.2022. - 27.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
apoptotic inducing factor (AIF), inner ear, yotari and wild-type mice, expression

Sažetak
DAB1-protein deficiency was investigated on the inner ear development of yotari in comparison to wild-type (wt) mice by expression of apoptotic inducing factor (AIF) at mice embryonic E13.5 and E15.5 in order to examine caspase independent apoptotic pathway. The spatial and temporal immunofluorescence expression pattern of AIF was determined by calculating area percentage covered by positive signal in the epithelium and mesenchyme of cochlear and semicircular ducts. Data were analyzed by t-test and were presented as a mean±SD. AIF expression in the epithelium of cochlear and semicircular duct were significantly higher in wt in comparison to yotari mice. The highest AIF expression was at E15.5 in the epithelium of cochlear duct, but this expression was twofold lower than in wt mice. AIF expression in mesenchyme of the cochlear and semicircular ducts were always statistically higher in wt in comparison to yotari, except for higher AIF expression in the cochlear duct of wt at E15.5 in comparison to yotari mice. Our results emphasize the relevance of AIF during development of vestibular and cochlear functions where they can serve as potential therapeutic targets in impairments of the inner ear.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA