Naslov
Immunohistochemical expression pattern of fgfr1, fgfr2 and rip5, in developing and postnatal kidneys of Dab1-/- (Yotari) mice

Autori
Kelam, Nela ; Racetin, Anita ; Kostić, Sandra ; Vukojević, Katarina ; Mardešić, Snježana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Abstracts of the 54th ESPN Annual Meeting, Ljubljana, Slovenia, in Pediatric Nephrology 2022 ; 37(11) / - , 2022, 2911-2911

Skup
54th ESPN Annual Meeting

Mjesto i datum
Ljubljana, Slovenija, 22.06.2022. - 25.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
yotari ; FGFR1 ; FGFR2 ; RIP5 ; CAKUT

Sažetak
Introduction: Yotari (Dab1-/-) mice are the autosomal recessive mutant mouse that emerged spontaneously during the generation of mice with a mutation in the gene encoding the inositol-1, 4, 5- trisphosphate receptor. Our latest research revealed the congenital anomalies of the kidney and urinary tract (CAKUT) phenotype culminating in renal hypoplasia followed by foot process effacement in the kidney glomeruli and functional decline kidney tissue of yotari. This study aimed to determine how functional silencing of the Dab1 gene affects the spatial and temporal patterns of expression of fibroblast growth factor receptor 1 (FGFR1), fibroblast growth factor receptor 2 (FGFR2), and receptor-interacting protein kinase 5 (RIP5) in the developing and postnatal kidneys of yotarimice as possible predictors of normal kidney formation and function. Material and methods: Animals were sacrificed on the gestation days E13.5 and E15.5 and postnatal days P4, P11, and P14. Analyses were performed using immunohistochemistry and fluorescent microscopy, followed by quantification of positive cells in kidney substructures. Data were analyzed by two- way ANOVA. Results: Dab1-/- animal kidneys exhibit decreased FGFR1/FGFR2 expression, suggesting the involvement of the observed markers in generating the CAKUT phenotype leading to renal hypoplasia. Between all developmental periods tested, there was no alteration in the immunoreactivity of RIP5 cells in the substructures of yotari kidneys. Conclusions: The abundant presence of observed proteins in kidneys and the dynamics of their expression found in this study suggest that FGFR1, FGFR2 and RIP5 play essential roles not only in early metanephric mesenchymal patterning, ureteric bud branching morphogenesis, nephrogenesis, and nephron progenitor survival but also in the maintenance of overall homeostasis and the maturation of kidney structures in the postnatal phase. Our results emphasize the crucial significance of the examined markers throughout normal kidney development and their potential involvement in renal pathology and diagnostics, where they could potentially serve as biomarkers and therapeutic targets.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA