Naslov
KIDNEY MORPHOLOGICAL CHANGES OF THE DAB1-/- MICE

Autori
Anita Racetin, Nela Kelam, Mirela Lozić, Natalija Filipović, Snježana Mardešić, Mirna Saraga-babić, Katarina Vukojević

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Skup
54th ESPN Annual Meeting

Mjesto i datum
Ljubljana, Slovenija, 22.06.2022. - 25.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Dab1, yotari, kidney morphological changes, CAKUT

Sažetak
Introduction: Examination of kidney size, nephron substructures diameters and podocyte morphology of the Dab1-/- (yotari) mice kidneys in comparison with wild type mice. We assumed that inactivation of Dab1 may cause the disorder in a broad spectrum of congenital anomalies of the kidney and urinary tract (CAKUT). Material and methods: Wild type and yotari mice were sacrificed at postnatal days P4, P11, and P14. Paraffin-embedded kidney tissues were sectioned and analyzed by bright-field and electron microscopy. Mean kidney and nephron substructures diameter was calculated and analyzed by statistical t-tests. Results: At the TEM microphotographs, foot process effacement in the glomeruli (G) of yotari mice was revealed, whereas aberrations in the structure of proximal convoluted tubules (PCT) and distal convoluted tubules (DCT) were not observed. Furthermore, yotari kidneys were smaller in size, with a reduced diameter of nephron segments, and thinner cortex. Conclusions: Renal hypoplasia in conjunction with foot process effacement revealed CAKUT phenotype and loss of functional kidney tissue of yotari.

Izvorni jezik
Engleski

POVEZANOST RADA