Naslov
Reactive oxygen species (ROS) confocal laser scanning imaging in TROL mutants

Autori
Dumančić, Ena ; Fulgosi, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Applied Bioinformatics in Life Sciences (4th edition) : Abstracts / - Leuven : VIB conference series, 2022, 78-78

Skup
Applied Bioinformatics in Life Sciences (4th edition)

Mjesto i datum
Leuven, Belgija, 10.03.2022. - 11.03.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Confocal scanning imaging

Sažetak
Photosynthesis is the process in photoautotrophic organisms that converts light energy into chemical energy (Walker, 2021). The very important part of this process is the transfer of electrons from reduced ferredoxin to NADP + , which is catalyzed by the flavoenzyme Ferredoxin- NADP + oxydoreductase (FNR) (Lintala, et al., 2012). Binding of the FNR to photosynthetic membranes of many vascular plants is assisted by the thylakoid rhodanase like protein (TROL) (Jurić, et al., 2009, Mulo, 2011). Within my PhD project I am examining influences of the TROL-FNR protein complex on electron transport and ROS scavenging, which indicates protective role of this complex. I will carry out detection and visualization of ROS using fluorescent probes (DHE for superoxides, SOSG for singlet oxygen molecules, spy-LHP for peroxides) for staining ROS molecules and confocal laser scanning microscopy technique for detecting them. This experiment will show presence and propagation of ROS molecules in chloroplasts and whole plant cells. Imaging is performed using a CCD camera. In my research I am using Arabidopsis thaliana (L.) Heynh as model plant, wild type (WT), TROL KO, ∆RHO mutants grown under normal humidity and in arid conditions. The main goal of these experiments is to demonstrate how TROL-FNR complex affects electron transport, ROS scavenging, management and distribution of high energy electrons in energetic processes of photosynthesis (Vojta et al., 2015) under normal soil humidity conditions and under abiotic stress conditions, in this case drought, and how deficiency of some components of this complex affects efficiency of the TROL-FNR pair. My research will contribute to the understanding of the behavior of plants under severe stress conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA