Naslov
Testing of candidate genes for compound biosynthesis in Arnica montana

Autori
Mrakovčić, Nikolina

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Mjesto
Zagreb, Hrvatska

Datum
27.02

Godina
2022

Stranica
100

Mentor
Bauer, Nataša

Ključne riječi
helenalin, sesquiterpene lactones, biosynthesis pathways, heterologous expression, Saccharomyces cereviasiae, germacrene A, germacrene A acid

Sažetak
Arnica montana L. is a traditional medicinal plant used for centuries. It owes its medicinal properties to sesquiterpene lactones, helenalin and dihydrohelenalin. Traditionally, the plants are harvested from its natural habitat, but there is need for alternative ways to sustainably obtain bioactive compounds. To achieve alternative production of A. montana bioactive sesquiterpene lactones, their biosynthesis pathway must be elucidated. Functional characterization of candidate genes, that might be involved in sesquiterpene lactones biosynthesis, has been conducted in the heterologous expression system yeast Saccharomyces cerevisiae and compounds analysis via gas chromatography-mass spectrometry. Additionally, analysis of potential candidate genes expression patterns and sesquiterpene lactones content distribution in plants was carried out. Candidate genes AmGAS and AmGAO were verified to encode respective germacrene A synthase and germacrene A acid oxidase, and their enzymatic activity was confirmed. Candidate genes AmCYPs were functionally analysed to a certain extent, with candidate AmCYP7 showing great potential for further investigation. Correlation of expression patterns and content of bioactive compounds in A. montana tissues revealed that among tested, AmCYP7 gene has potential to be part of sesquiterpene lactones biosynthesis pathway and provided preliminary evidence for their biosynthesis at different developmental stages during the plant´s life cycle

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA