Naslov
Twist of the mitotic spindle culminates at anaphase onset and depends on microtubule- associated proteins along with external forces

Autori
Trupinić, Monika ; Kokanović, Barbara ; Ponjavić, Ivana ; Wadsworth, Patricia ; Fritz-Laylin, Lillian ; Tolić, Iva M.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
Mitotic spindle: From living and synthetic systems to theory

Mjesto i datum
Split, Hrvatska, 28.03.2021. - 31.03.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Mitotic spindle ; Mitosis ; Chirality ; Twist ; Torques ; Motor proteins ; Kinesins ; Augmin

Sažetak
In addition to linear forces, rotational forces are present in the mitotic spindle, reflected in the left-handed twisted shapes of microtubule bundles that make the spindle chiral [1]. However, the molecular origins of spindle chirality are unknown. Here we show that spindles are most twisted at the beginning of anaphase, and reveal multiple molecular players involved in spindle chirality. Inhibition of Eg5/kinesin-5 in a non- cancer cell line abolished spindle twist and depletion of Kif18A/kinesin-8 resulted in a right- handed twist. Depletion of the crosslinker PRC1 also resulted in a right-handed twist, but overexpression of PRC1 abolished twist. Interestingly, depletion of augmin led to a right- handed twist. Round spindles were more twisted than elongated ones, a notion that we directly tested by compressing the spindle along its axis, which resulted in stronger left-handed twist. Finally, we show that spindle chirality is present in other organisms, specifically in the amoeba Naegleria gruberi. Although this organism has a spindle that is different from those in human somatic cells, microtubule bundles still adopt a helical form. Surprisingly, bundles in amoebas typically follow a right-handed helical path. We conclude that spindle twist is controlled by multiple molecular mechanisms acting at different locations within the spindle as well as forces.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

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