Naslov
Comparison of Zn content in rapid-acting insulin and biphasic suspension by FAAS

Autori
Shkunnikova, Sofia ; Širac, Tea ; Lalić, Katarina ; Kulišić, Ankica ; Jablan, Jasna

Izvornik
Croatica chemica acta (0011-1643) 94 (2021), 2; 95-102

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
zinc ; rapid-acting and intermediate-acting insulins ; flame atomic absorption spectrometry

Sažetak
Various insulin analogs (rapid-acting and intermediate-acting insulin) have been investigated for the determination of zinc content by flame atomic absorption spectrometry. This paper presents the validation of a method and comparison of zinc content in the insulin samples studied. The method was linear r2 = 0.9997), the limit of detection was 0.0098 mg L–1 and the limit of quantification was 0.0296 mg L– 1, the precision (as relative standard deviation) was up to 7.4 %, and the accuracy was within a range of 95.6 % to 100.1 % for the recovery of fortified insulin samples. The zinc content in the insulin samples ranged from 14.9 mg L–1 to 16.3 mg L–1 for rapid-acting insulin and 18.7 mg L–1 to 19.9 mg L– 1 for intermediate-acting insulin. A higher zinc content was found in the intermediate-acting insulins than in the rapid-acting insulin analogs (p < 0.05). The obtained results should be considered in the establishment of new or improvement of currently available procedures used to assure the quality, safety and efficacy of insulin products.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA