Naslov
PROTEOLYTIC PROCESSING AND COVALENT BINDING OF YEAST CELL WALL PROTEIN Scw10

Autori
Lozančić, Mateja ; Grbavac, Antonija ; Matičević, Ana ; Hrestak, Dora ; Teparić, Renata ; Mrša, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDBMB22 From Science to Knowledge / Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2022, 101-101

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology: From Science to Knowledge (HDBMB22)

Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Kex2, yapsins, Scw4, Scw10, cell wall

Sažetak
The proteins Scw4, Scw10, and Scw11 form a group of putative glucanases found in the yeast cell wall. SCW4 and SCW10 are paralogous genes with very high sequence identity. It has been previously shown that Scw4 undergoes complex proteolytic processing that affects its biological function. Scw4 is first processed by the Kex2 protease in the Golgi and then by yapsin proteases located in the cell membrane and/or cell wall. This two-step processing has been reported to affect its activity and cell wall binding. Scw10 is known to have a Kex2 recognition site, but its proteolytic processing has not been thoroughly investigated. It has also been shown that both Scw4 and Scw10 can form non-covalent and covalent bonds with the wall. The covalent bond is of particular interest because it differs from other previously known bonds in the cell wall. Although it has similar properties to the bonds formed by Pir family proteins, both Scw4 and Scw10 lack the typical Pir binding sequence. Therefore, the binding sequence and mechanism of Scw4 and Scw10 remain unknown. Here we present results obtained by studying the processing of Scw10. They show that the processing is similar in complexity to that of Scw4 but differs in terms of processing sites. The role of several putative Scw4/Scw10 binding sequences in forming a covalent bond to cell wall structures has been discussed.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA