Naslov
Biomimetička kromatografija: Učinkovit alat za procjenu bioraspoloživosti aloina i aloe- modina
(Biomimetic chromatography: Effective tool for bioavailability evaluation of aloin and aloe-emodin)

Autori
Jeličić, Mario-Livio ; Kovačić, Jelena ; Amidžić Klarić, Daniela ; Mornar, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts: 19th Ružićka days - Today science-tomorrow industry / Babić, Jurislav - Osijek : Zagreb : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2022, 144-145

Skup
19. Ružičkini dani "Danas znanost - sutra industrija"

Mjesto i datum
Vukovar, Hrvatska, 21.09.2022. - 23.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
botanike, upalne bolesti crijeva, biomimetička kromatografija
(botanicals, inflammatory bowel disease, biomimetic chromatography)

Sažetak
Aloe vera (syn.: Aloe barbadensis Miller) is the most commercialized Aloe species belonging to the Xanthorrhoeaceae family. Aloin is the major anthraquinone of aloe and characterized as the C- glycoside of aloe-emodin. Due to polyphenolic structure, aloin and aloe-emodin are considered as physiologically active compounds responsible for aloe biological effects such as reduction of inflammation. Despite the frequent use of aloe, limited information is available for their bioavailability which is a very common phenomenon for other phytochmicals as well. A study providing deeper mechanistic insightsinto the bioavailability of aloin and aloe-emodin is essential to develop better treatment strategies in the future based on botanical products. Although traditional evaluation of drugs bioavailability and performance in human body require long lasting and expensive clinical studies, a more affordable and faster approach is being developed. Innovative biomimetic chromatographic methods have shown to be good alternative for such studies. The aim of this work was to evaluate parameters such as hydrophobicity, lipophilicity, and plasma protein binding using chromatographic tools, especially growing population of biomimetic columns mimicking cell membranes, as well as ones containing plasma proteins (human serum albumin (HSA) and alpha-1- acid glycoprotein (AGP). As expected, aloin as glycoside showed lower hydrophobicity (RM0=1.85, logk0=1.37) compared to aloe-emodin (RM0=3.06, logk0=3.01). On the other hand, aloin showed superior affinity to phospholipids compared to aloe-emodin (Δlogk0 IAM=1.68). Both compounds showed the higher affinity for HSA (62.7% aloin ; 97.5% aloe-emodin) than AGP (44.1% aloin ; 84.2% aloe-emodin).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

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