Pregled bibliografske jedinice broj: 1218397
Development and validation of an HPLC-MS / MS multi-class method with special emphasis on pharmacologically active agents in milk and chicken feed
Development and validation of an HPLC-MS / MS multi-class method with special emphasis on pharmacologically active agents in milk and chicken feed, 2021., diplomski rad, diplomski, Prehrambeno-tehnološki fakultet Osijek, Osijek
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Naslov
Development and validation of an HPLC-MS / MS
multi-class method with special emphasis on
pharmacologically active agents in milk and
chicken feed
(Development and validation of an HPLC-MS / MS
multi-class method with special emphasis on
pharmacologically active agents in milk and
chicken feed (Razvoj i validacija HPLC-MS/MS
metode za višeklasnu analizu s posebnim naglaskom
na farmakološki aktivne tvari u mlijeku i hrani za
perad))
Autori
Kenjerić, Lidija
Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski
Fakultet
Prehrambeno-tehnološki fakultet Osijek
Mjesto
Osijek
Datum
16.07
Godina
2021
Stranica
53
Mentor
Klapec, Tomislav
Neposredni voditelj
Sulyok, Michael
Ključne riječi
veterinary drug residues ; HPLC-MS/MS ; poultry feed ; milk ; validation
Sažetak
In this work, a multi-class high-pressure liquid chromatography - tandem mass spectrometry (HPLC-MS / MS) method was developed and validated for the analysis of pharmacologically active substances, i.e., different classes of veterinary drug residues in milk and poultry feed. The method used in this study was a modification of the approach described by Malachová et al. (2014). The m/z ratio for all optimized analytes were detected both in the ESI positive and negative mode and the more sensitive mode was selected. The m/z ratio for all optimized analytes was determined in both positive and negative ionization modes. Optimized parameters have been implemented in a "new" multimethod. The stability of standards was tested, and different solvents were tested for the purpose of optimizing the extraction procedure. Prior to analysis, milk and poultry feed samples were subjected to extraction under acidic and neutral extraction conditions. The method was validated through examination of the following performance parameters: linearity, LOD, LOQ, precision, accuracy and within laboratory repeatability. The results of the method validation show that despite the large range of tested analytes in terms of polarity, stability and solubility, a large part of the analytes (80- 90%) meet the SANTE validation criteria.
Izvorni jezik
Engleski
Znanstvena područja
Prehrambena tehnologija, Nutricionizam
