Naslov
Embryonic development effects of emamectin benzoate on the brittle star Amphiura filiformis and the sea urchin Brissopsis lyrifera

Autori
Rodríguez-Satizábal, Simón ; Dupont, Sam ; Grđan, Sanja ; Schmelzer, Melina ; Vigliano Relva, Julieta ; Gomes, Tânia ; Lilicrap, Adam ; Macken, Ailbhe

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
PRIMO 21 Conference Book / - , 2022, 100-100

Skup
21st International Symposium on Pollutant Responses In Marine Organisms (PRIMO21)

Mjesto i datum
Göteborg, Švedska, 22.05.2022. - 25.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
aquaculture ; veterinary medicines ; emamectin benzoate ; embryotoxicity

Sažetak
In 2019, the salmon industry in Norway represented 94% of the country’s total aquaculture production, with a value of 68 billion NOK and 1.4 million tons of fish produced. The aquaculture industry can be affected by biofouling and diseases, for which different types of antifouling compounds and veterinary medicinal products (VMP) have been developed. To control sea lice, iVMPs have been used and some of these substances can enter the water or remain uneaten by the fish (from chemicals dosed through feed) and can accumulate on the seabed. The avermectin emamectin benzoate (EMB) is one of these VMPs that was initially developed as a crop insecticide. The main objective of this study was thus to assess the effects of EMB on the embryonic development of two sediment dwelling benthic organisms, the brittle star (Amphiura filiformis) and the sea urchin (Brissopsis lyrifera), which play a major ecological role in the community. Organisms were collected using a Van Veen grab (30 m) and a trawl (60 m) for the brittle star and the sea urchin, respectively. Brittle star spawning was induced by photoperiod and gametes were collected for fertilization. The sea urchin spawning was induced using an intracelomic injection of 0.5 M KCl. Afterwards, sperm and eggs were collected for the fertilization process. For both species different quality standard verifications were made including: sperm mobility, egg shape and gamete compatibility. Using 6 concentrations of EMB (0.001, 0.01, 0.1, 1, 10 and 100 µg/L), a negative (only seawater) and a solvent control (0.01% methanol), fertilized eggs (1300 eggs/ml per bottle) were exposed for 120 hours. Every day a sample (5 ml/replicate) was extracted from each bottle and after adding a Paraformaldehyde (4%) solution, the eggs/larvae were counted and abnormalities registered using a microscope. For the first 72 hours of exposure, length was measured, while on hours 96 and 120, body length, body rod length and arm length were recorded. Embryonic development effects of EMB were analyzed and included survival, growth, skeletal development and body symmetry. Data analysis is ongoing, but preliminary results point to effects on growth and body symmetry at 100 µg/L for both species. These results are part of the ANTIVENOM project which is assessing the effects of antifoulants and VMPs used in the salmon farming.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne biotehničke znanosti

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