Naslov
Assessing the chronic effects of emamectin benzoate and teflubenzuron on the embryonic development of Amphiura filiformis

Autori
Rodríguez-Satizábal, Simón ; Dupont, Sam ; Grđan, Sanja ; Schmelzer, Melina ; Vigliano Relva, Julieta ; Gomes, Tânia ; Lillicrap, Adam ; Macken, Ailbhe

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9th Norwegian Environmental Toxicology Symposium: Towards a Clean Ocean: Abstract Book / - , 2022, 69-69

Skup
9th Norwegian Environmental Toxicology Symposium: Towards a Clean Ocean

Mjesto i datum
Tromsø, Norveška, 08.06.2022. - 10.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
aquaculture ; veterinary medicines ; emamectin benzoate ; teflubenzuron ; embryo toxicity

Sažetak
The aquaculture industry is important in Norway, being the second largest export income to the country economy. Within the species farmed, salmon represented 94% of the total production in 2019. Salmon culture can be affected by different diseases including parasite infestations, mainly from sea lice. To prevent this, industry has developed veterinary medicinal products (VMP) such as emamectin benzoate (EMA) and teflubenzuron (TEF) to treat fish. These substances are persistent in marine sediments (>120 and 170 days, respectively), so it is important to know their effects on non-target organisms such as keystone species that contribute to processes such as bioturbation and bioengineering. In the North Sea, the brittle star Amphiura filiformis plays a major ecological role as an ecosystem engineer. The main objective of this study was to assess the effects of EMA and TEF on the brittlestar A. filiformis. Organisms were collected near the Kristineberg Marine Station (Sweden) in August 2021 using a trawl (60 m). The spawning was induced by photoperiod and subsequently the gametes were collected for the fertilization process. Different quality verifications were made prior to the experiments, including egg quality, sperm mobility and finally gamete compatibility. After checking the fertilization success, fertilized eggs (1300 eggs per bottle) were exposed for 5 days. For both substances, 6 concentrations, plus a negative (seawater) and a solvent control (0.01 % methanol) were used. For the daily sampling, a 5 ml (replicate) sample was extracted from each bottle. Abnormalities and morphometric analyses were performed using a microscope. For the first 3 days of exposure, only body length was measured, and on days 4 and 5, body rod length and arm length were also recorded in addition to body length. Effects on survival, growth, skeletal development and body symmetry were analyzed for both substances. Data analysis is ongoing but preliminary results indicate effects on growth and body symmetry at the highest concentrations for both compounds. These results are part of the ANTIVENOM project which is assessing the effects of antifoulants and VMPs used in the salmon fishing industry in Norway.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne biotehničke znanosti

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