Naslov
Dynamics of specialized metabolism and physiological responses of grapevine (Vitis vinifera) infected with flavescence dorée phytoplasma

Autori
Davosir, Dino

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet, Biološki odsjek

Mjesto
Zagreb

Datum
26.09

Godina
2022

Stranica
107

Mentor
Šeruga Musić, Martina ; Šola, Ivana

Ključne riječi
effectors ; FDp ; phenolics ; plant-pathogen interactions

Sažetak
Phytoplasmas (genus ‘Candidatus Phytoplasma’) are intracellular parasitic bacteria and pathogens of numerous plants. Flavescence dorée phytoplasma (FDp) causes severe damage to viticultural production in Europe and is a quarantine pathogen in EU. In this study, symptomatic and asymptomatic grapevine (Vitis vinifera L.) var. ‘Pinot gris’ leaves were sampled at three time points through the development of infection. FDp was detected by using triplex real-time PCR and genotyped by sequencing and phylogenetic analysis of map gene which identified two genotypes, M38 and M54. By using spectrophotometric and chromatographic (RP- HPLC, GC-MS) analyses, impact of two FDp genotypes on grapevine physiological parameters and phenolic metabolism was evaluated through the development of infection. Genotype M54 impacted physiological parameters less severely, while maintaining higher titre than M38. Correlation analysis revealed that changes in physiological parameters could be linked to FDp titre. Content of all main branches of phenolic metabolism and most individual compounds had similar trends, pointing to the general induction of the stem phenolic biosynthetic pathway by FDp infection, particularly in the last time point. Analysed phytoplasma genotype-dependant changes to grapevine physiology provide data on certain targets of FDp in grapevine and could assist the identification of potential specific effectors of this phytoplasma.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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