Altered Expression of EMT-Related Factors Snail, Wnt4, and Notch2 in the Short-Term Streptozotocin-Induced Diabetic Rat Kidneys

Dragun Jurić, Matea; Racetin, Anita; Filipović, Natalija; Kelam, Nela; Kostić, Sandra; Ljutić, Dragan; Vukojević, Katarina

doi:10.3390/life12101486

Pregled bibliografske jedinice broj: 1216861

Altered Expression of EMT-Related Factors Snail, Wnt4, and Notch2 in the Short-Term Streptozotocin-Induced Diabetic Rat Kidneys

Dragun Jurić, Matea; Racetin, Anita; Filipović, Natalija; Kelam, Nela; Kostić, Sandra; Ljutić, Dragan; Vukojević, Katarina

Altered Expression of EMT-Related Factors Snail, Wnt4, and Notch2 in the Short-Term Streptozotocin-Induced Diabetic Rat Kidneys // Life, 12 (2022), 10; 1486, 14 doi:10.3390/life12101486 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1216861 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Altered Expression of EMT-Related Factors Snail, Wnt4, and Notch2 in the Short-Term Streptozotocin-Induced Diabetic Rat Kidneys

Autori
Dragun Jurić, Matea ; Racetin, Anita ; Filipović, Natalija ; Kelam, Nela ; Kostić, Sandra ; Ljutić, Dragan ; Vukojević, Katarina

Izvornik
Life (2075-1729) 12 (2022), 10; 1486, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diabetic nephropathy ; epithelial to mesenchymal transition (EMT) ; snail ; Notch2 ; Wnt4

Sažetak
Background: The aim of this study was to determine the expression of epithelial to mesenchymal transition (EMT)-related transcription factors Snail, Wnt4, and Notch2 with key roles in renal fibrosis, in different renal areas of diabetic rats: glomeruli (G), proximal and distal convoluted tubules (PCT ; DCT). Methods: Male Sprague Dawley rats were instilled with 55 mg/kg streptozotocin (diabetes mellitus type I model, DM group) or citrate buffer (control group). Kidney samples were collected 2 weeks and 2 months after DM induction and processed for immunohistochemistry. Results: Diabetic animals showed higher Wnt4 kidney expression both 2 weeks and 2 months post-DM induction, while Snail expression significantly increased only 2 weeks after DM initiation (p < 0.0001). We determined significantly higher expression of examined EMT-related genes in different kidney regions in diabetic animals compared with controls. The most substantial differences were observed in tubular epithelial cells in the period of 2 weeks after induction, with higher Snail and Wnt4 expression in PCT and increased Snail and Notch2 expression in DCT of diabetic animals (p < 0.0001 ; p < 0.001). Conclusion: The obtained results point to the EMT-related factors Snail, Wnt4, and Notch2 as a potential contributor to diabetic nephropathy development and progression. Changes in their expression, especially in PCT and DCT, could serve as diagnostic biomarkers for the early stages of DM and might be a promising novel therapeutic target in this condition.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA

Ustanove:
KBC Split,
Medicinski fakultet, Split

Profili:

Katarina Vukojević (autor)