Naslov
The effect of pulse corticosteroid therapy on peripheral blood lymphocyte apoptosis in patients with relapsing-remitting multiple sclerosis - preliminary results

Autori
Petravić, Damir ; Zurak, Niko ; Petelin, Željka ; Mahović, Darija ; Golemović, Mirna ; Franjić, Jasna ; Ribarić, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 2nd Dubrovnik International Conference on Multiple Sclerosis and Continuing Education ; u: Neurologia Croatica. Supplement (ISSN 1331-5196) 52 (2003) (S1) / - , 2003, 57-57

Skup
Dubrovnik International Conference on Multiple Sclerosis and Continuing Education (2 ; 2003)

Mjesto i datum
Dubrovnik, Hrvatska, 21.05.2003. - 24.05.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
apoptosis; corticosteroid therapy; relapsing-remitting multiple sclerosis

Sažetak
The aim of the study was to assess the expression of CD95 molecule on peripheral blood (PB) CD4+ and CD8+ T-lymphocytes in patients with relapsing-remitting multiple sclerosis (RRMS) in the relapse of the disease, immediately before and after pulse corticosteroid therapy with regard to the presumed mechanism of corticosteroid effect on the induction of apoptosis of autoreactive peripheral blood lymphocytes. The study included 8 patients (5F, 3M) with RRMS diagnosed by McDonald's criteria. Mean age of patients was 34.88&#177; 4.1 years and mean duration of the disease 3.5&#177; 4.97 years. The analysis of T-cells was done in the relapse of the disease, immediately before and after pulse corticosteroid therapy consisting of intravenous methylprednisolone 1000 mg/day in 500 ml physiologic saline in short infusions over five days. The proportions of T-lymphocytes and CD95+ T-lymphocyte subsets were analysed by use of monoclonal antibodies (anti-CD4-PE/Cy5, anti-CD8-FITC and anti-CD95-PE, DAKO) and flow cytometry (FACSscan cytometer and CellQuest software, Becton Dickinson). On statistical analysis nonparametric Wilcoxon test for dependent samples was used. Results were presented as median and range. A p value <0.05 was considered statistically significant. The proportion of CD8+ T-lymphocytes was significantly higher immediately before pulse corticosteroid therapy (M=37.65% ; range 33.3%-48.5%) in comparison to the period after corticosteroid therapy (M=28.7% ; range 22.1%-41.5%, p=0.012). The CD4/CD8 ratio was significantly lower before (M=1.08 ; range 0.64-1.44), than after (M=1.44 ; range 0.8-2.45, p=0.017) pulse corticosteroid therapy. The proportion of CD8+ T-lymphocytes bearing CD95 was significantly higher immediately before (M=31.6% ; range 25.4%-39.1%), than after (M=20.45% ; range 15%-30%, p=0.012) pulse corticosteroid therapy. The proportions of CD4+ T-lymphocytes and CD4+CD95+ T-lymphocytes were not significantly different before and after pulse corticosteroid therapy. The change of CD95 expression on peripheral blood CD8+ T-lymphocytes shows that pulse corticosteroid therapy influences on apoptosis of autoreactive lymphocytes in patients with RRMS.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA