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Pregled bibliografske jedinice broj: 1215803

Ion mobility mass spectrometry reveals rare sialylated glycosphingolipid structures in human cerebrospinal fluid

Sarbu, Mirela; Fabris, Dragana; Vukelić, Željka; Clemmer, David E.; Zamfir, Alina D.
Ion mobility mass spectrometry reveals rare sialylated glycosphingolipid structures in human cerebrospinal fluid // Molecules, 27 (2022), 3; 743, 13 doi:10.3390/molecules27030743 (međunarodna recenzija, članak, znanstveni)

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Naslov
Ion mobility mass spectrometry reveals rare sialylated glycosphingolipid structures in human cerebrospinal fluid

Autori
Sarbu, Mirela ; Fabris, Dragana ; Vukelić, Željka ; Clemmer, David E. ; Zamfir, Alina D.

Izvornik
Molecules (1420-3049) 27 (2022), 3; 743, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ion mobility separation mass spectrometry (IMS MS) ; sialylated glycosphingolipids ; gangliosides ; human cerebrospinal fluid ; collision-induced dissociation ; GalNAc-GD1c isomer

Sažetak
Gangliosides (GGs) represent an important class of biomolecules associated with the central nervous system (CNS). In view of their special role at a CNS level, GGs are valuable diagnostic markers and prospective therapeutic agents. By ion mobility separation mass spectrometry (IMS MS), recently implemented by us in the investigation of human CNS gangliosidome, we previously discovered a similarity between GG profiles in CSF and the brain. Based on these findings, we developed IMS tandem MS (MS/MS) to characterize rare human CSF glycoforms, with a potential biomarker role. To investigate the oligosaccharide and ceramide structures, the ions detected following IMS MS separation were submitted to structural analysis by collision-induced dissociation (CID) MS/MS in the transfer cell. The IMS evidence on only one mobility feature, together with the diagnostic fragment ions, allowed the unequivocal identification of isomers in the CSF. Hence, by IMS MS/MS, GalNAc-GD1c(d18:1/18:1) and GalNAc- GD1c(d18:1/18:0) having both Neu5Ac residues and GalNAc attached to the external galactose were for the first time discovered and structurally characterized. The present results demonstrate the high potential of IMS MS/MS for biomarker discovery and characterization in body fluids, and the perspectives of method implementation in clinical analyses targeting the early diagnosis of CNS diseases through molecular fingerprints.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Željka Vukelić (autor)

Avatar Url Dragana Fabris (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Sarbu, Mirela; Fabris, Dragana; Vukelić, Željka; Clemmer, David E.; Zamfir, Alina D.
Ion mobility mass spectrometry reveals rare sialylated glycosphingolipid structures in human cerebrospinal fluid // Molecules, 27 (2022), 3; 743, 13 doi:10.3390/molecules27030743 (međunarodna recenzija, članak, znanstveni)
Sarbu, M., Fabris, D., Vukelić, Ž., Clemmer, D. & Zamfir, A. (2022) Ion mobility mass spectrometry reveals rare sialylated glycosphingolipid structures in human cerebrospinal fluid. Molecules, 27 (3), 743, 13 doi:10.3390/molecules27030743.
@article{article, author = {Sarbu, Mirela and Fabris, Dragana and Vukeli\'{c}, \v{Z}eljka and Clemmer, David E. and Zamfir, Alina D.}, year = {2022}, pages = {13}, DOI = {10.3390/molecules27030743}, chapter = {743}, keywords = {ion mobility separation mass spectrometry (IMS MS), sialylated glycosphingolipids, gangliosides, human cerebrospinal fluid, collision-induced dissociation, GalNAc-GD1c isomer}, journal = {Molecules}, doi = {10.3390/molecules27030743}, volume = {27}, number = {3}, issn = {1420-3049}, title = {Ion mobility mass spectrometry reveals rare sialylated glycosphingolipid structures in human cerebrospinal fluid}, keyword = {ion mobility separation mass spectrometry (IMS MS), sialylated glycosphingolipids, gangliosides, human cerebrospinal fluid, collision-induced dissociation, GalNAc-GD1c isomer}, chapternumber = {743} }
@article{article, author = {Sarbu, Mirela and Fabris, Dragana and Vukeli\'{c}, \v{Z}eljka and Clemmer, David E. and Zamfir, Alina D.}, year = {2022}, pages = {13}, DOI = {10.3390/molecules27030743}, chapter = {743}, keywords = {ion mobility separation mass spectrometry (IMS MS), sialylated glycosphingolipids, gangliosides, human cerebrospinal fluid, collision-induced dissociation, GalNAc-GD1c isomer}, journal = {Molecules}, doi = {10.3390/molecules27030743}, volume = {27}, number = {3}, issn = {1420-3049}, title = {Ion mobility mass spectrometry reveals rare sialylated glycosphingolipid structures in human cerebrospinal fluid}, keyword = {ion mobility separation mass spectrometry (IMS MS), sialylated glycosphingolipids, gangliosides, human cerebrospinal fluid, collision-induced dissociation, GalNAc-GD1c isomer}, chapternumber = {743} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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