Pregled bibliografske jedinice broj: 1212626
Study on the Surface Interactions of Co(II) with Phospholipids from the Marine Environment
Study on the Surface Interactions of Co(II) with Phospholipids from the Marine Environment // Journal of marine science and engineering, 10 (2022), 9; 1261-1273 doi:10.3390/ jmse10091261 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1212626 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Study on the Surface Interactions of Co(II) with
Phospholipids from the Marine Environment
Autori
Bačinić, Anđela ; Vukosav, Petra ; Kero, Ivana ; Mlakar, Marina
Izvornik
Journal of marine science and engineering (2077-1312) 10
(2022), 9;
1261-1273
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
: cobalt(II) ; 1, 10-Phenanthroline ; phospholipids ; voltammetry ; atomic force microscopy
Sažetak
Natural phospholipid (PL)n extract from cultured green algae Dunaliela tertiolecta was used as the main component of cell membranes for studies on the interaction with trace metal Co(II). The phospholipids of the membranes were extracted from other biological components using TiO2 -µSPE cartridges selective for the phosphate group according to a completely new protocol. The interaction of Co(II) with natural and standard phospholipids, phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylglycerol (PG) are registered only in the presence of the additional chelating ligand 1, 10-Phenathroline (Phen). 1, 10-Phenathroline, as a model of humic substances in the marine environment, formed a neutral complex with Co(II) by the substitution of water molecules of central metal ions. The interaction of hydrophobic mixed-ligand complexes with phospholipids was enabled by the substitution of the remaining water molecules in the coordination shell of Co(II), which was registered by voltammetric measurements. The Co(II)-Phen-PL complex is reduced from the adsorbed state at −1.65 V by the transfer of two electrons, followed by its irreversible dissociation and desorption, indicating an EC mechanism. The interaction between the mixed-ligand complexes Co(II)-Phen-lipids was confirmed by atomic force microscopy (AFM). AFM images of PL, PL with Phen mixture and PL, Phen with Co(II) showed different 3D structures on the mica surface, indicating changes caused by the interaction between cobalt(II), 1, 10- Phenanthroline and phospholipids.
Izvorni jezik
Engleski
Znanstvena područja
Interdisciplinarne prirodne znanosti
POVEZANOST RADA
Projekti:
HRZZ-IP-2018-01-3105 - Biokemijski odgovori površinskog sloja oligotrofnog područja Jadranskog mora na atmosfersko taloženje (BiREADI) (Frka Milosavljević, Sanja, HRZZ - 2018-01) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
