Naslov
Algal cell surface properties and behaviour as stress markers for aquatic ecosystems

Autori
Novosel, Nives ; Ivošević DeNardis, Nadica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6. simpozij studenata doktorskih studija PMF-a : knjiga sažetaka = 6th Faculty of Science PhD student symposium : book of abstracts / Schneider, Petra - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2022, 82-83

ISBN
978-953-6076-93-2

Skup
6. Simpozij studenata doktorskih studija PMF-a = 6th Faculty of Science PhD Student Symposium

Mjesto i datum
Zagreb, Hrvatska, 23.04.2022. - 24.04.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
abiotic stressors ; cell behaviour ; microalgae ; surface properties

Sažetak
Microalgae represent the most sensitive part of the trophic food chain and therefore reliably and rapidly indicate climate changes in aquatic ecosystems. The aim of this work was to link the response of algal cells in terms of their surface properties and behavior to individual abiotic stressors to better understand the survival and adaptation strategies of cells in an aquatic ecosystem. Three widely distributed algal species with varying cell barrier complexity were cultured at selected temperatures and salinities to provoke stress. The response of the cells was studied in terms of growth dynamics, surface properties, physiological activity, and motility behavior using the surface methods and open-source software ICY. The results show that all cell species successfully adapted to the selected conditions. A decrease in temperature and salinity triggered a chemical, mechanical and behavioral response in the microalgae. All cell species become stiffer, cell movement is minimal, and they differ in their hydrophobicity and physiological activity [1-4]. Algae surrounded by a glycocalyx layer appear to be temperature sensitive and salt tolerant, while algae surrounded by calcite-coated theca behave in the opposite manner. Algae surrounded by an organosilicate wall seem to adapt well to both stressors. The results obtained show that algal cells exhibit detectable, species-specific, and stressor-dependent changes that can be related to the structural properties of the cell envelope. This study has shown that stressor-dependent surface properties and behavior provide insights into understanding algal adaptation mechanisms at the single-cell level, that can be used to indicate ecosystem disturbances, algal blooms and find appropriate commercial applications. ACKNOWLEDGMENT This work is supported by the Croatian Science Foundation Project “From algal cell surface properties to stress markers for aquatic ecosystems” (IP-2018-01-5840). REFERENCES [1] N. Novosel, D. Kasum, P. Žutinić, T. Legović, N. Ivošević DeNardis, J. Appl. Phycol. 32 (2020) 4057-4067. [2] N. Novosel, N. Ivošević DeNardis, Electroanalysis 33 (2021) 1433-1446. [3] N. Novosel, T. Mišić Radić, J. Zemla, M. Lekka, A. Čačković, D. Kasum, T. Legović, P. Žutinić, M. Gligora Udovič, N. Ivošević DeNardis, J. Appl. Phycol. 34 (2021) 243-259. [4] N. Novosel, T. Mišić Radić, M. Levak Zorinc, J. Zemla, M. Lekka, I. Vrana, B. Gašparović, L. Horvat, D. Kasum, T. Legović, P. Žutinić, M. Gligora Udovič, N. Ivošević DeNardis, J. Appl. Phycol. (2022), under minor revision.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti

POVEZANOST RADA