Naslov
Cathepsin D intratumoral heterogeneity and its prognostic significance in laryngeal SCC. Prognostic significance of cathepsin D intratumoral heterogeneity in laryngeal SCC

Autori
Ćorić, M. ; Ilić, Ivana ; Batelja, L. ; Bumber, Željko ; Bura, Miljenko ; Seiwerth, Sven

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
19 th European Congress of Pathology

Mjesto i datum
Ljubljana, Slovenija, 06.11.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cathepsin D; laryngeal SCC

Sažetak
Our previous investigations demonstrated prognostic significance for cathepsin D immunoreactivity in laryngeal SCC. We also demonstrated heterogeneity for AgNOR and DNA content in different areas of LSCC showing prognostic potential for DNA content of invasive tumor border. As this heterogeneity is a potential source of highly variable results we decided to investigate cathepsin D parameters in different microscopically defined tumor areas of LSCC. Patients and methods: 100 total laryngectomy specimen have been immunostained with anti-cathepsin D antibody (DAKO). Three distinct regions were analyzed: transformation zone, invasive tumor margin and tumor center. Immunoreactivity in tumor and stromal cells was accessed by scoring (0 - 4) in at least 200 cells respectively. Tumor and stromal cell positivity was accessed in the most positive area of tumor staining of the respective tumor region. Results and conclusion. Significant difference of immunostaining was observed between tumor invasive margin and the other regions. In most cases the invasive margin was the most prominently stained region for tumor as well as for stromal cells. Most patients showed stronger staining of stromal than tumoral cells. Only patients with survival less than 30 months showed stronger tumor than stromal cell staining in invasive margin. From this we can conclude that the basis for cathepsin D prognostic value in laryngeal SCC lies in the tumor/stromal cell correlation of cathepsin D immunoreactivity of the invasive tumor margin.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA