Naslov
Moderate perinatal hypoxia causes permanent reorganization of the hippocampal perineuronal nets and interneurons network in rat

Autori
Trnski, Sara ; Ilić, Katarina ; Bobić-Rasonja, Mihaela ; Banovac, Ivan ; Drlje, Matea ; Darmopil, Sanja ; Petanjek, Zdravko ; Jovanov-Milošević, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Xjenza, 10 (2022), Special issue / - , 2022, 162-163

Skup
8th Mediterranean Neuroscience Society Conference (MNS)

Mjesto i datum
Dubrovnik, Hrvatska, 29.05.2022. - 02.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
extracellular matrix ; chondroitin sulfate proteoglycans ; perineuronal nets ; parvalbumin ; plasticity ; hyperactivity ; aggrecan ; versican ; neurocan

Sažetak
The study investigates the possibility, extent, and persistence of structural or connectivity changes in the rat hippocampus after moderate perinatal hypoxia. The distribution, qualitative and quantitative changes in parvalbumin-positive neurons (PV) and the perineuronal nets (PNN) were researched. For this purpose, 18 Wistar Han (RccHan: WIST) rat pups were randomly subjected to hypoxia (partial pressure of oxygen-pO2 73 mmHg ; atmosphere pressure-pATM 350 mmHg) or normoxia (pO2 159 mmHg ; pATM 760 mmHg) for two hours at the first postnatal day. At the age of 3.5 months, the rats were sacrificed, their brains were isolated and used for immunohistochemistry and analysis of PV and PNN of the hippocampi (bregma: levels -2.92 mm ; -3.36 mm ; -4.56 mm) using OlympusBX60 and Neurolucida 10 software. Qualitative analysis revealed differences in the co-expression pattern of PNNs’ constituents (aggrecan, versican, and neurocan) and differences in PNN and PV areal/layer distribution between hypoxia and normoxia groups. Quantitative analysis revealed a significantly higher density of PNN and PV, especially in the CA1 area of the hippocampus of mature animals subjected perinatally to hypoxia. These results align with the changes in behavior such as significant hyperactivity and a slower pace of learning in rats after perinatal hypoxia, published previously by our group. Further research is needed to disclose the underlying sequence of molecular events and the injury- plasticity relations that lead to the permanent hippocampal and behavioral alteration in rats after moderate perinatal hypoxia. Funding: project CSF-IP-2019-04-3182 and project GA KK01.1.1.01.0007 by the European Union through the European Regional Development Fund.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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