Naslov
Drug resistance analysis of Mycobacterium tuberculosis by means of drug susceptibility test and reverse hybridization-based line probe assay

Autori
Katalinić Janković, Vera ; Milutin, Nina ; Obrovac, Mihaela ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final programme and Abstracts of the 24th Annual Congress of the European Society of Mycrobiology / Krüner, Annika - Tartu : Frens Conference Services, 2003, 59-59

Skup
24th Annual Congress of the European Society of Mycrobiology

Mjesto i datum
Tartu, Estonija, 29.06.2003. - 02.07.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Mycobacterium tuberculosis; Drug resistance

Sažetak
Tuberculosis (TB) is the leading infectious cause of death worldwide. Croatia takes place among the countries with still high incidence of tuberculosis (33/100, 000 in 2002). Several countries around the world have registered outbreaks of resistance and even multidrug resistance (MDR) TB. Croatia has a low incidence of anti-TB drug resistance (3%), and 0.6% of MDR. MDR status is considered resistance to at least rifampicin (major first line anti-TB drug) and isoniazid, with or without resistance to other drugs. Genetic modification of TB associated to rifampicin resistance is one of the fist precursor events of MDR. The objective of this study was to compare the classical drug susceptibility test (DST – proportion method on Loewenstein-Jensen medium) and the reverse hybridization-based line probe assay (INNO-LiPA Rif.TB, Innogenetics, Belgium). The DST enables the differentiation for established resistance to rifampicin, isoniazid, ethambutol and streptomycin, while INNO-LiPA Rif.TB enables the recognition of early genetic mutation of rifampicin resistance region of the beta-subunit of the RNA polymerase (rpoB). Herein, we examined 39 M. tuberculosis strains of which 1 sensitive and 2 resistant only to rifampicin, both by DST and INNO-LiPA Rif.TB. Among 20 rifampicin and other anti-TB drugs resistant (MDR) strains by DST, INNO-LiPA Rif.TB allowed the identification of 19 strains resistant to rifampicin, of which one was identified as mixture of the mutant strain (R4a) and the wild type (WT), while the remaining one was identified as only WT. Fourteen out of 16 samples resistant to anti-TB drugs other than rifampicin were confirmed by INNO-LiPA Rif.TB, while 2 were identified as resistant to rifampicin. The overall concordance of the two tests represents 92.3%. DST is very informative assay on TB drug resistance but it is time consuming and the interpretation of the results could be ambiguous contrary to INNO-LiPA Rif.TB. Beside that, INNO-LiPA Rif.TB assay is very reliable and simple to perform. Moreover, it allows the identification of genetic mutation before the appearance of phenotypic changes of the bacteria. Thus, it could be considered as the screening test for drug resistance for M. tuberculosis strains.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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