Naslov
Distribution of microbes involved in methane cycle along the salinity gradient

Autori
Selak, Lorena ; Šparica Miko, Martina ; Matijaković Mlinarić, Nives ; Orlić, Sandi

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEMS Conference on Microbiology Belgrade 2022 / - , 2022, 1-1

Skup
2nd FEMS Conference on Microbiology

Mjesto i datum
Beograd, Srbija, 30.06.2022. - 03.07.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
16S rRNA sequencing, surface sediments microbiology, methane cycle

Sažetak
Background: By being the second most abundant greenhouse gas in the atmosphere with much higher retentive capacity than carbon dioxide, methane and its genesis by methanogens are being studied to reduce its emission into the atmosphere, thus mitigating global warming. Shallow lake ecosystems are one of the hot spots in natural global methane emissions. The crucial role in regulating the amount of methane exiting the ecosystem have methanotrophs, and their occurrence depends on environmental conditions. Objectives: The study’s goal was to determine the spatial distribution of the most abundant methane cycle contributors in lake sediments of the coastal Lake Vrana. Since the lake is known to experience salinity intrusions via karstified ridge and subsurface canal Jugovir, we wanted to investigate whether salinity affects the microbial community involved in the methane cycle. Methods: Surface sediments were collected, of which seven represented the longitudinal transect of the lake while the rest were taken at the canal inlets or bays. Pore water ion concentrations and organic carbon and total nitrogen percentage were determined. 16S diversity was determined on the Illumina platform. Results: Detected methanotrophs belonged to five different orders, with the sulfate-dependent anaerobic ANME-1b being predominantly abundant in the entire lake, except on stations one and twelve, where aerobic methanotrophs Methylococcales were dominant. The most abundant order of methanogens was Methanomicrobiales with dominant genus Methanolinea, while detected sulfate reducers belonged to Desulfatiglandales, Desulfobaccales, and Desulfobacterales. Microbial community composition was influenced by sulfate concentrations, salinity, nitrogen compounds, and organic carbon.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA