Pregled bibliografske jedinice broj: 120340
Colony PCR amplification of actinomycete DNA
Colony PCR amplification of actinomycete DNA // Journal of antibiotics, 56 (2003), 4; 423-424 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 120340 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Colony PCR amplification of actinomycete DNA
Autori
Gathogo, W.N. Esther, Waugh C.W. Alastair ; Perić, Nataša ; Redpath, B. ; Maria, Long, F. Paul
Izvornik
Journal of antibiotics (0021-8820) 56
(2003), 4;
423-424
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Actinomycetes; high G+C content DNA; colony PCR amplification; polyketide biosynthesis
Sažetak
This report describes colony PCR amplification of high G+C content DNA from a range of actinomycete genera. This method describes the use of a non-ionic surfactant to efficiently promote lysis of the actinomycete cell walls and enhance DNA polymerase activity. This approach allows the rapid identification of different genera by the amplification of 16S rRNA gene sequences. In addition, we also demonstrated the identification of strains carrying DNA encoding proteins associated with specific metabolic pathways, for example, in polyketide biosynthesis.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
POVEZANOST RADA
Projekti:
0058008
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb
Profili:
Nataša Perić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
