Naslov
No association between BRAF V600E mutations and aggressive clinicopathologic features in Croatian patients with papillary thyroid carcinoma

Autori
Šamija, Ivan ; Periša, Josipa ; Blažeković, Ivan ; Romić, Matija ; Regović Džombeta, Tihana ; Granić, Roko ; Jukić, Tomislav ; Fröbe, Ana ; Kusić, Zvonko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
EACR 2022 / - , 2022

Skup
EACR 2022 Annual Congress of the European Association for Cancer Research

Mjesto i datum
Sevilla, Španjolska, 20.06.2022. - 23.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
papillary thyroid cancer ; BRAF mutations ; metastatic status ; prognostic markers

Sažetak
BRAF V600E mutations are the most common genetic alteration in patients with papillary thyroid carcinoma. Their prognostic value and association with aggressive features in patients with papillary thyroid carcinoma have been extensively studied but with conflicting results. The aim of this study was to examine association of BRAF mutations with aggressive clinicopathologic features in a cohort of Croatian patients with papillary thyroid carcinoma. In this study we analysed thyroid papillary cancer samples from 177 patients. DNA was isolated from formalin-fixed paraffin-embedded tissue and assessed for presence of BRAF V600E mutation using competitive allele- specific TaqMan polymerase chain reaction. Association between BRAF mutations and aggressive clinicopathologic features was examined using appropriate statistical test (Chi-square, Fisher’s exact or Mann-whitney test). This research was funded by Croatian Science Foundation project IP- 2019-04-1130. BRAF V600E mutation was found in 110 (62%) out of 177 patients. Patients with BRAF mutation were significantly older than patients without BRAF mutation (median age 50 vs. 39 years ; p=0.0004). BRAF mutation was found in 52 (66%) out of 79 patients with localised disease, 42 (63%) out of 67 patients with lymph node metastases, and in 16 (52%) out of 31 patients with distant metastases. There was no significant association between BRAF mutations and metastatic status (p=0.197). We also found no statistically significant association between presence of BRAF V600E mutation and gender (p=0.128), tumour diameter (p=0.404), capsular invasion (p=0.496), intrathyroidal dissemination (p=0.641), extrathyroidal extension (p=0.911), lymph node metastases (p=0.330), and distant metastases (p=0.097). Our study showed no association between BRAF V600E mutation and clinicopathologic features of aggressive papillary thyroid carcinoma.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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