Naslov
COMPARISON OF MITOTIC INDEX, GROWTH FRACTION (EVALUATED BY KI-67), ER, PR, CATHEPSIN D, P53, AND BCL-2 STATUS BETWEEN PRIMARY BREAST CANCER AND CORRESPONDING AXILLARY METASTATIC SITES.

Autori
Dmitrović, Branko ; Kristek, Jozo ; Kurbel, Sven

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Virchows Archiv Sept 2003 / - , 2003

Skup
19th European Congress of Pathology

Mjesto i datum
Ljubljana, Slovenija, 06.09.2003. - 11.09.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Bcl-2; breast cancer; cathepsin D; estrogen receptors; Ki-67 antigen; metastatic; mitotic index; p53; primary; progesterone receptors

Sažetak
BACKGROUND: It has been postulated that the equilibrium of different clones within a tumor is eventually overcome by a biologically dominant one with enhanced metastatic potential. Therefore, the metastatic tumor should be composed of more aggressive tumor cell clones than the primary one. We analyzed mitotic index, proliferation antigen Ki-67, estrogen receptors (ER), progesterone receptors (PR), cathepsin D activity, protein p53, and Bcl-2 oncoprotein status in order to explore the assumed differences in the clonal composition between the primary breast cancer and axillary metastasis. MATERIAL AND METHODS: ER, PR, p53, Bcl-2, Ki-67, and cathepsin D statuses in 60 patients with the primary ductal invasive carcinoma of the breast and at least one axillary metastatic lesion were analyzed by immunohistochemistry (IHC &#8211 ; DAKO LSAB&#61666 ; 2 System). Mitotic index was assessed by counting the number of mitoses in primary and metastatic lesions. Statistical analysis included t-test for dependent samples, linear correlation analysis, and ROC-curve based contingency table analysis. RESULTS: We found higher mitotic index (p=0.018, r=0.51), higher growth fraction (Ki-67, p=0, 045, r=0.71), and higher level of overexpression of Bcl-2 (p=0.014, r=0.86) in axillary metastatic lesion than in primary breast tumor.. There were no significant differences in ER (p=0.16, r=0.98), PR (p=0.08, r=0.87), cathepsin D (p=0, 92, r=-0.06), and p53 (p=0.36, r=0.87) status. Higher mitotic and Ki-67 rates were associated with lower ER and PR expression, whereas higher cathepsin D level was associated with lower Bcl-2 expression, both in primary tumor and metastasis. CONCLUSIONS: Between the paired primary tumor and axillary metastatic lesions, samples had concordant ER, PR, p53, and cathepsin D status when analyzed by IHC. Tumor clone with enhanced metastatic potential might be characterized with higher mitotic index and overexpression of both Ki-67 antigen and Bcl-2 oncoprotein.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA