Naslov
Influence of subinhibitory concentrations of ceftazidime, ciprofloxacin and azithromycin on the morphology and adherence of P-fimbriated escherichia coli

Autori
Vraneš, Jasmina ; Žagar, Živojin ; Kurbel, Sven

Izvornik
Journal of chemotherapy (1120-009X) 8 (1996), 4; 254-260

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Animal Anti-Infective Agents ; pharmacology Azithromycin ; pharmacology Bacterial Adhesion ; drug effects Ceftazidime ; pharmacology Cell Line Cercopithecus aethiops Ciprofloxacin ; pharmacology Escherichia coli ; drug effects/ultrastructure ; Fimbriae ; Bacterial Ki

Sažetak
The influence of subinhibitory concentrations (1/2, 1/4, 1/8, 1/16 and 1/32 x MIC) of ceftazidime, ciprofloxacin and azithromycin on the morphology and adherence of 29 wild-type P- fimbriated strains of Escherichia coli was studied. Bacterial adherence to the Buffalo green monkey (BGM) cell line was tested before and after treatment with antibiotics and detected by means of an immunofluorescence staining. Significant dose dependent reduction of bacterial adherence was observed, which correlated with the alterations in bacterial cell morphology. After exposure of strains to sub-MICs of antibiotics, normal shapes, spherical forms and filaments were noted. The greatest filamentation and the greatest loss of adherence ability occurred at 1/2 x MIC of ceftazidime. Treatment with sub-MICs of ciprofloxacin resulted in shorter filaments, while filamentation did not occur after bacterial exposure to sub-MICs of azithromycin. Azithromycin was least damaging to the adherence ability of E. coli and at a concentration of 1/2 x MIC caused globoid cell formation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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