Naslov
Influence of consumption of eggs enriched with functional compounds on endothelial function in healthy young subjects

Autori
Šušnjara, Petar ; Mihaljević, Zrinka ; Stupin, Ana ; Kolobarić, Nikolina ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
27. međunarodno savjetovanje KRMIVA = 27th international conference KRMIVA

Mjesto i datum
Opatija, Hrvatska, 01.06.2022. - 03.06.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
microcirculation, endothelium, vasodilation, cyclooxygenases, postocclusive hyperemic response, functional food, hen eggs

Sažetak
Functional food, in addition to the appropriate nutritional effects, also has a beneficial effect on one or more targeted functions in the body, it improves health and wellbeing or reduces the risk of disease. Recent research within the Scientific Center of Excellence for Personalized Health Care has shown that consumption of n-3 polyunsaturated fatty acids (n-3 PUFAs) - fortified eggs had a positive effect on microvascular reactivity, and the level of oxidative stress and has mild anti- inflammatory effects in healthy young subjects of both sexes. The next step in functional food research is to further examine eggs enriched with other nutrients. The aim of this study was to determine the effect of consumption of hen eggs enriched with four nutrients (n-3 PUFA, lutein, vitamin E and selenium) on microvascular reactivity in healthy young subjects and to assess potential role of metabolites of COX1 and COX2 in that response. This was a randomised, double- blind, placebo-controlled interventional study (part of ID: NCT04564690, the study was registered on the clinicaltrial.gov). Subjects were divided in Control group (n=14 ; consumed regular hen eggs (approximately 1.785 mg of vitamin E, 0.330 mg of lutein, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily), and Nutri4 group (n=20) who consumed enriched eggs (approximately 3.29 mg of vitamin E, 1.85 mg of lutein, 0.06 mg of selenium and 1026 mg of n-3 PUFAs daily), 3 eggs a day for 3 weeks. Skin microvascular blood flow in response to post- occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine and sodium nitroprusside (endothelium-independent) was assessed by laser Doppler flowmetry before and after dietary protocols. In separate sets of experiments, participants took perorally a 100 mg of indomethacin (a non-selective cyclooxygenase inhibitor) before functional (LDF) measurements. Protein expression of enzymes important in microvascular reactivity (COX1, COX2, iNOS, eNOS and nNOS) was assessed from peripheral blood mononuclear cells (PBMC) by Western blot before and after respective dietary protocol. Consumption of functionally enriched eggs improved endothelium-dependent vasodilation which was eliminated by indomethacin. The observed changes may be attributed to altered production of vasoactive metabolites by COX enzyme to which increased expression.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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