Naslov
Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging

Autori
Planinc, Ivo ; Garcia-Canadilla, Patricia ; Dejea, Hector ; Ilić, Ivana ; Guasch, Eduard ; Zamora, Monica ; Crispi, Fatima ; Stampanoni, Marco ; Miličić, Davor ; Bijnens, Bart ; Bonnin, Anne ; Čikeš, Maja

Izvornik
Scientific reports (2045-2322) 11 (2021); 14020, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cardiac tissue infarction ; tomography ; platform ; orientation ; extraction ; failure ; driven ; fiber ; scar

Sažetak
Cardiovascular research is in an ongoing quest for a superior imaging method to integrate gross- anatomical information with microanatomy, combined with quantifiable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of different myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two configurations resulting in 5.8 µm and 0.65 µm effective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantification of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantified by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantification of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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