Naslov
Effects of Teflubenzuron on the Early Development of the Sea Urchin Brissopsis Lyrifera and the Brittlestar Amphiura filiformis

Autori
Rodríguez-Satizábal, Simon ; Dupont, Sam ; Grđan, Sanja ; Schmelzer, Melina ; Vigliano Relva, Julieta ; Gomes, Tania ; Lilicrap, Adam ; Macken, Ailbhe

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book SETAC Europe 32nd Annual Meeting / - , 2022, 496-497

Skup
32nd Annual Meeting: Towards a reduced pollution society (SETAC 2022)

Mjesto i datum
Kopenhagen, Danska, 15.05.2022. - 19.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
aquaculture, veterinary medicines, teflubenzuron, fertilization

Sažetak
The salmonid aquaculture industry can be affected by biofouling and diseases. Different types of antifouling compounds and veterinary medicinal products (VMP), have been used to control biofouling and parasites such as sea lice. Teflubenzuron (TEF) is one VMP that has historically been used in Norway, but it is very persistent (half-life of 170 days in sediment) and has potentially toxic effects on non-target organisms. The main objective of this study was to assess the effects of TEF on two benthic organisms, representative of the Nordic environment. The sea urchin Brissopsis lyrifera was chosen because it can alter sediment grain distribution, while the brittlestar Amphiura filiformis is a burrowing organism that uses its arms for suspended feeding. Organisms were collected near the Kristineberg Marine Station (Sweden) in August 2021 using a Van Veen grab (30 m) and a trawl (60 m) for the brittlestar and sea urchin, respectively. Sea urchins were spawned using an intracelomic injection of 0.5 M KCl (ca.1 ml). Subsequently, eggs and sperm were collected for fertilization. Spawning in the brittlestars was induced by photoperiod and gametes were collected for fertilization. Prior to fertilization, sperm mobility, egg quality and gamete compatibility were evaluated for both species. After checking the fertilization success, fertilized eggs (1300 eggs/ml per bottle) were exposed for 5 days to 6 concentrations of TEF (0.001, 0.01, 0.1, 1, 10 and 100 μg/L), alongside a negative (just seawater) and a solvent control (0.01 % methanol). A 5 ml (replicate) sample was extracted daily from each bottle, 2-4 drops of Paraformaldehyde (4%) solution added and the number of eggs/larvae were counted for abnormalities and morphometric analysis using a microscope. On the first 3 days of exposure, length was measured, while on days 4 and 5, body length, body rod length and arm length were recorded. Effects on survival, growth, skeletal development and body symmetry of TEF were analyzed. Data analysis is ongoing but preliminary results point to effects on growth and body symmetry at 100 μg/L for both species. These results are part of the ANTIVENOM project which is assessing the effects of antifoulants and VMPs used in the salmon fishing industry in Norway.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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