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Pregled bibliografske jedinice broj: 1193324

Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification

Rodriguez-Temporal, David; Alcaide, Fernando; Mareković, Ivana; O’Connor, James Anthony; Gorton, Rebecca; van Ingen, Jakko; Van den Bossche, An; Héry-Arnaud, Genevieve; Beauruelle, Clémence; Orth-Höller, Dorothea et al.
Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification // Scientific reports, 12 (2022), 1; 1237, 7 doi:10.1038/s41598-022-05315-7 (međunarodna recenzija, članak, znanstveni)

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Naslov
Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification

Autori
Rodriguez-Temporal, David ; Alcaide, Fernando ; Mareković, Ivana ; O’Connor, James Anthony ; Gorton, Rebecca ; van Ingen, Jakko ; Van den Bossche, An ; Héry-Arnaud, Genevieve ; Beauruelle, Clémence ; Orth-Höller, Dorothea ; Palacios- Gutiérrez, Juan-José ; Tudó, Griselda ; Bou, Germán ; Ceyssens, Pieter-Jan ; Garrigó, Montserrat ; González-Martin, Julià ; Greub, Gilbert ; Hrabak, Jaroslav ; Ingebretsen, André ; Mediavilla-Gradolph, Maria Concepción ; Oviaño, Marina ; Palop, Begoña ; Pranada, Arthur B. ; Quiroga, Lidia ; Ruiz-Serrano, Maria Jesús ; Rodríguez-Sánchez, Belén

Izvornik
Scientific reports (2045-2322) 12 (2022), 1; 1237, 7

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
MALDI-TOF MS, identification, nontuberculous mycobacteria

Sažetak
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species- level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA

Ustanove:
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb

Profili:

Avatar Url Ivana Mareković (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Rodriguez-Temporal, David; Alcaide, Fernando; Mareković, Ivana; O’Connor, James Anthony; Gorton, Rebecca; van Ingen, Jakko; Van den Bossche, An; Héry-Arnaud, Genevieve; Beauruelle, Clémence; Orth-Höller, Dorothea et al.
Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification // Scientific reports, 12 (2022), 1; 1237, 7 doi:10.1038/s41598-022-05315-7 (međunarodna recenzija, članak, znanstveni)
Rodriguez-Temporal, D., Alcaide, F., Mareković, I., O’Connor, J., Gorton, R., van Ingen, J., Van den Bossche, A., Héry-Arnaud, G., Beauruelle, C. & Orth-Höller, D. (2022) Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification. Scientific reports, 12 (1), 1237, 7 doi:10.1038/s41598-022-05315-7.
@article{article, author = {Rodriguez-Temporal, David and Alcaide, Fernando and Marekovi\'{c}, Ivana and O’Connor, James Anthony and Gorton, Rebecca and van Ingen, Jakko and Van den Bossche, An and H\'{e}ry-Arnaud, Genevieve and Beauruelle, Cl\'{e}mence and Orth-H\"{o}ller, Dorothea and Palacios- Guti\'{e}rrez, Juan-Jos\'{e} and Tud\'{o}, Griselda and Bou, Germ\'{a}n and Ceyssens, Pieter-Jan and Garrig\'{o}, Montserrat and Gonz\'{a}lez-Martin, Juli\`{a} and Greub, Gilbert and Hrabak, Jaroslav and Ingebretsen, Andr\'{e} and Mediavilla-Gradolph, Maria Concepci\'{o}n and Ovia\~{n}o, Marina and Palop, Bego\~{n}a and Pranada, Arthur B. and Quiroga, Lidia and Ruiz-Serrano, Maria Jes\'{u}s and Rodr\'{\i}guez-S\'{a}nchez, Bel\'{e}n}, year = {2022}, pages = {7}, DOI = {10.1038/s41598-022-05315-7}, chapter = {1237}, keywords = {MALDI-TOF MS, identification, nontuberculous mycobacteria}, journal = {Scientific reports}, doi = {10.1038/s41598-022-05315-7}, volume = {12}, number = {1}, issn = {2045-2322}, title = {Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification}, keyword = {MALDI-TOF MS, identification, nontuberculous mycobacteria}, chapternumber = {1237} }
@article{article, author = {Rodriguez-Temporal, David and Alcaide, Fernando and Marekovi\'{c}, Ivana and O’Connor, James Anthony and Gorton, Rebecca and van Ingen, Jakko and Van den Bossche, An and H\'{e}ry-Arnaud, Genevieve and Beauruelle, Cl\'{e}mence and Orth-H\"{o}ller, Dorothea and Palacios- Guti\'{e}rrez, Juan-Jos\'{e} and Tud\'{o}, Griselda and Bou, Germ\'{a}n and Ceyssens, Pieter-Jan and Garrig\'{o}, Montserrat and Gonz\'{a}lez-Martin, Juli\`{a} and Greub, Gilbert and Hrabak, Jaroslav and Ingebretsen, Andr\'{e} and Mediavilla-Gradolph, Maria Concepci\'{o}n and Ovia\~{n}o, Marina and Palop, Bego\~{n}a and Pranada, Arthur B. and Quiroga, Lidia and Ruiz-Serrano, Maria Jes\'{u}s and Rodr\'{\i}guez-S\'{a}nchez, Bel\'{e}n}, year = {2022}, pages = {7}, DOI = {10.1038/s41598-022-05315-7}, chapter = {1237}, keywords = {MALDI-TOF MS, identification, nontuberculous mycobacteria}, journal = {Scientific reports}, doi = {10.1038/s41598-022-05315-7}, volume = {12}, number = {1}, issn = {2045-2322}, title = {Multicentre study on the reproducibility of MALDI- TOF MS for nontuberculous mycobacteria identification}, keyword = {MALDI-TOF MS, identification, nontuberculous mycobacteria}, chapternumber = {1237} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • Social Science Citation Index (SSCI)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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