Naslov
Genetic relatedness among basil (Ocimum spp.) accessions using RAPD markers

Autori
Šatović, Zlatko ; Liber, Zlatko ; Kolak, Ivan ; Karlović, Ksenija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 1st Croatian Congress on Molecular Life Sciences / Kućan, Željko - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2002

Skup
1st Croatian Congress on Molecular Life Sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Ocimum spp.; RAPDs; genetic relatedness

Sažetak
Genetic relatedness among basil accessions including six species of Ocimum (O. basilicum L., O. americanum L., O. x citriodorum L., O. minimum L., O. gratissiumum L., O. tenuiflorum L.) and six botanical varieties or cultivars of O. basilicum L. (var. basilicum L. cv. Genovese, var. basilicum L. cv. Sweet Basil, var. difforme Benth., var. purpurascens Benth., cv. Dark Opal, and var. thyrsiflorum /L./ Benth.) were analyzed by random amplified polymorphic DNA (RAPD) markers. Five primers were used for amplification and yielded a total of 102 easily scorable polymorphic markers. Jaccard indices were calculated and phylogenetic relationships were determined by neighbor joining cluster analysis. Different Ocimum species were clearly divided into separate clusters with the exception of O. minimum accessions that clustered together with O. basilicum accessions. In addition to morphological, chemical and crossability data, RAPD analysis can be a useful tool for resolving existing problems in identification and classification of basils.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA