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Pregled bibliografske jedinice broj: 1191329

Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS- CoV-2 for Use in Hospital Emergency Departments

Pavletić, Martina; Mazor, Marija; Lerga, Mate; Mileta, Tatjana; Železnjak, Jelena; Ružić, Tina; Ravlić, Sanda; Palčevski, Dora; Kirinčić, Jelena; Mežnarić, Silvestar et al.
Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS- CoV-2 for Use in Hospital Emergency Departments // Viruses, 13 (2021), 12; 2413, 17 doi:10.3390/v13122413 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1191329 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS- CoV-2 for Use in Hospital Emergency Departments

Autori
Pavletić, Martina ; Mazor, Marija ; Lerga, Mate ; Mileta, Tatjana ; Železnjak, Jelena ; Ružić, Tina ; Ravlić, Sanda ; Palčevski, Dora ; Kirinčić, Jelena ; Mežnarić, Silvestar ; Mišković, Ana ; Materljan, Maja ; Šustić, Alan ; Lisnić, Berislav ; Juranić Lisnić, Vanda

Izvornik
Viruses (1999-4915) 13 (2021), 12; 2413, 17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
SARS-CoV-2 ; qPCR ; direct qPCR ; dqPCR ; point-of-care PCR ; emergency department ; molecular diagnostics ; COVID-19

Sažetak
During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10, 000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near- patient settings and can be performed by medical personnel with little prior experience in qPCR.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Projekti:
HRZZ-IP-CORONA-IP-CORONA-2020-04-2053 - Stvaranje preduvjeta za uvođenje SARS-CoV-2 seroterapije u Hrvatskoj (Halassy, Beata, HRZZ - 2020-04) ( CroRIS)
EK-KK.01.1.1.01.0006 - Jačanje kapaciteta CERVIRVAC-a za istraživanja u virusnoj imunologiji i vakcinologiji (Jonjić, Stipan, EK ) ( CroRIS)

Ustanove:
Medicinski fakultet, Rijeka,
Klinički bolnički centar Rijeka,
Sveučilište u Zagrebu

Profili:

Avatar Url Vanda Juranić Lisnić (autor)

Avatar Url Berislav Lisnić (autor)

Avatar Url Sanda Ravlić (autor)

Avatar Url Alan Šustić (autor)

Avatar Url Jelena Železnjak (autor)

Avatar Url Marija Mazor (autor)

Avatar Url Martina Pavletić (autor)

Poveznice na cjeloviti tekst rada:

doi www.mdpi.com

Citiraj ovu publikaciju:

Pavletić, Martina; Mazor, Marija; Lerga, Mate; Mileta, Tatjana; Železnjak, Jelena; Ružić, Tina; Ravlić, Sanda; Palčevski, Dora; Kirinčić, Jelena; Mežnarić, Silvestar et al.
Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS- CoV-2 for Use in Hospital Emergency Departments // Viruses, 13 (2021), 12; 2413, 17 doi:10.3390/v13122413 (međunarodna recenzija, članak, znanstveni)
Pavletić, M., Mazor, M., Lerga, M., Mileta, T., Železnjak, J., Ružić, T., Ravlić, S., Palčevski, D., Kirinčić, J. & Mežnarić, S. (2021) Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS- CoV-2 for Use in Hospital Emergency Departments. Viruses, 13 (12), 2413, 17 doi:10.3390/v13122413.
@article{article, author = {Pavleti\'{c}, Martina and Mazor, Marija and Lerga, Mate and Mileta, Tatjana and \v{Z}eleznjak, Jelena and Ru\v{z}i\'{c}, Tina and Ravli\'{c}, Sanda and Pal\v{c}evski, Dora and Kirin\v{c}i\'{c}, Jelena and Me\v{z}nari\'{c}, Silvestar and Mi\v{s}kovi\'{c}, Ana and Materljan, Maja and \v{S}usti\'{c}, Alan and Lisni\'{c}, Berislav and Jurani\'{c} Lisni\'{c}, Vanda}, year = {2021}, pages = {17}, DOI = {10.3390/v13122413}, chapter = {2413}, keywords = {SARS-CoV-2, qPCR, direct qPCR, dqPCR, point-of-care PCR, emergency department, molecular diagnostics, COVID-19}, journal = {Viruses}, doi = {10.3390/v13122413}, volume = {13}, number = {12}, issn = {1999-4915}, title = {Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS- CoV-2 for Use in Hospital Emergency Departments}, keyword = {SARS-CoV-2, qPCR, direct qPCR, dqPCR, point-of-care PCR, emergency department, molecular diagnostics, COVID-19}, chapternumber = {2413} }
@article{article, author = {Pavleti\'{c}, Martina and Mazor, Marija and Lerga, Mate and Mileta, Tatjana and \v{Z}eleznjak, Jelena and Ru\v{z}i\'{c}, Tina and Ravli\'{c}, Sanda and Pal\v{c}evski, Dora and Kirin\v{c}i\'{c}, Jelena and Me\v{z}nari\'{c}, Silvestar and Mi\v{s}kovi\'{c}, Ana and Materljan, Maja and \v{S}usti\'{c}, Alan and Lisni\'{c}, Berislav and Jurani\'{c} Lisni\'{c}, Vanda}, year = {2021}, pages = {17}, DOI = {10.3390/v13122413}, chapter = {2413}, keywords = {SARS-CoV-2, qPCR, direct qPCR, dqPCR, point-of-care PCR, emergency department, molecular diagnostics, COVID-19}, journal = {Viruses}, doi = {10.3390/v13122413}, volume = {13}, number = {12}, issn = {1999-4915}, title = {Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS- CoV-2 for Use in Hospital Emergency Departments}, keyword = {SARS-CoV-2, qPCR, direct qPCR, dqPCR, point-of-care PCR, emergency department, molecular diagnostics, COVID-19}, chapternumber = {2413} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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