Naslov
Ion Channels Modulation in Sprague-Dawley Rats on a High Salt Intake – Changes in Flow-induced Dilation Mechanisms

Autori
Lukačević, Ivana ; Mihaljević, Zrinka ; Kozina, Nataša ; Stupin, Ana, Drenjančević, Ines ; Jukić Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th International Student and 17th Bridges in Life Sciences Conferences - Book of Abstract / - , 2022, 32-32 / - , 2022, 32-32

ISBN
978-615-6006-03-5

Skup
4th International Student; 17th Bridges in Life Sciences Conferences

Mjesto i datum
Prag, Češka Republika, 06.04.2022. - 09.04.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Ion Channels, Middle Cerebral Artery, Sprague-Dawley Rats

Sažetak
Introduction: High salt (HS) intake leads to endothelial dysfunction and reduced vascular responses to different physiological stimuli in both, humans and animal models. This study aimed to asses the role of ion channels in flow-induced dilation (FID) in isolated middle cerebral arteries (MCAs) of Sprague-Dawley rats on HS diet. Methods: Male, 11-weeks-old male rats (N=6- 10/group) were divided in: CTRL (control group of rats) and HS (4% NaCl in rodent chow fed for 1 week), water ad libidum. Rats were anesthetized with ketamine-chloride (75 mg/kg) and midazolam (0.5 mg/kg), decapitated and MCA was isolated and cannulated on glass pipettes (Myograph Pressure System Model 110P MyoView Version 1.2.0 DMT ; Danish Myo Technology). Reactivity of MCAs in response to stepwise increases in pressure gradients (Δ10-Δ100 mmHg) was determined in absence/presence of agonist and antagonists, respectively, for large conductance Ca2+-activated K+- channel (KCa2+, NS-1619 and iberiotoxin) ; ATP- sensitive potassium channels (KATP, cromakalim and glibenclamide) and TRPV4 (GSK1016790A and RN-1734). Results: FID was reduced in HS compared with CTRL group. Each of applied antagonists reduced FID at each pressure gradient in CTRL group, whereas in HS group impaired FID at Δ20 and Δ40 mmHg, and at high pressure gradient (Δ80 and Δ100 mmHg) only RN-1734 reduced FID. In CTRL group, each of applied agonists has no effect on FID, whereas in HS group, each of them restored FID at each pressure gradients. Discussion: HS diet causes impaired responses to FID. In control group KCa2+, KATP and TRPV4 ion channels have role in FID mechanisms. In HS group KCa2+, KATP and TRPV4 channels have role in FID mechanisms only at pressure gradients of Δ20 and Δ40 mmHg, whereas at higher pressure gradients (Δ80 and Δ100) only TRPV4 has role. Conclusion: Results suggest that HS dietry intake affect the mechanisms of FID by modulating ion channels.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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