Naslov
Characterization of Verticillium dahliae isolates from olive and susceptibility of local olive cultivars to Verticillium wilt in Istria, Croatia

Autori
Godena, Sara ; Ivić, Dario ; Ban, Dean ; Goreta Ban, Smiljana

Izvornik
Scientia horticulturae (0304-4238) 292 (2022); 110630, 7

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Olea europaea L., Verticillium wilt, Cultivars, Chlorophyll content, Disease resistance

Sažetak
Verticillium wilt, caused by Verticillium dahliae is considered the most serious biotic threat to olive trees. The disease is detected in almost all regions where olive is cultivated, and it may cause olive decline and dieback. The aim of this study was to determine the incidence and distribution of Verticillium wilt on olive trees in Istria (Croatia), to identify pathotype groups and race structure of the local V. dahliae population and to determine the susceptibility of Croatian olive cultivars to Verticillium wilt. In a field survey, Verticillium wilt was found to be the most common cause of olive decline, as it was detected in 61% of investigated locations. Out of 78 isolates of potentially pathogenic fungi obtained from olive trees with decline or dieback symptoms, 36% isolates of V. dahliae were determined. Molecular identifications based on PCR with specific primer sets showed that all V. dahliae isolates belonged to the nondefoliating pathotype (ND) and race 1. Olive cultivars Buza, Karbonaca, Istarska Bjelica, Rosinjola and Leccino were selected for susceptibility experiments, performed in a greenhouse, with a mix of three indigenous ND race 1 V. dahliae isolates used as inoculum. Different susceptibilities of local olive cultivars to V. dahliae infection were observed, with cultivar Karbonaca scoring as the most resistant. To the best of our knowledge, this is the first study showing Verticillium wilt as a widespread olive disease in Istria, confirming for the first time in Croatia the presence of a less aggressive, nondefoliating pathotype and race 1.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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