Naslov
Identification and characterization of human eosinophil cationic protein by an epitope-specific antibody

Autori
Boix, Ester ; Carreras, Esther ; Nikolovski, Zoran ; Cuchillo, Claudi M. ; and Nogues M. Victoria

Izvornik
Journal of leukocyte biology (0741-5400) 69 (2001), 6; 1027-1035

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
immunoassay , granulocytes , plasma , sputum

Sažetak
The eosinophil cationic protein (ECP) is a basic secretion protein involved in the immune response system. ECP levels in biological fluids are an indicator of eosinophil-specific activation and degranulation and are currently used for the clinical monitoring and diagnosis of inflammatory disorders. A polyclonal epitope-specific antibody has been obtained by immunizing rabbits with a conjugated synthetic peptide. A sequence corresponding to a large exposed loop in the human ECP threedimensional structure (D115–Y122) was selected as a putative antigenic epitope. The antibody was purified on an affinity column using recombinant ECP (rECP) as antigen. The antibody (D112–P123 Ab) specifically recognizes rECP and its nativeglycosylated and nonglycosylated forms in plasma, granulocytes, and sputum. The antibody detects as little as 1 ng of rECP, can be used both in reducing and nonreducing conditions, and doesnot cross-react with the highly homologous eosinophil-derived neurotoxin or other proteins of the pancreatic ribonuclease superfamily.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA