Naslov
Quantitative Evaluation of 2D-Chromatograms with CCD Camera

Autori
Petrović, Mira ; Kaštelan-Macan, Marija ; Babić, Sandra

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the 10th International Symposium on Instrumental Planar Chromatography / Nyredy, S. ; Szabady, B. ; Kaiser, R.E. ; Studer, A. - Budakalasz : Research Institute for Medicinal Plants, 1998, 117-125

Skup
The 10th International Symposium on Instrumental Planar Chromatography

Mjesto i datum
Visegrád, Mađarska, 16.05.1998. - 19.05.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
two-dimensional TLC; CCD camera; quantitative analysis

Sažetak
A mixture of 10 pesticides: MCPA, atrazine, propham, chloropropham, ofurace, boyleton, boycon A, boycon B, tetramethrin and alpha-cypermethrin was separated on CN-precoated HPTLC plates using polar mobile phase (methanol-water) in 1st and nonpolar mobile phase (hexane-diethyl ether) in 2nd direction. Chromatograms were recorded with sensitive colour CCD camera and evaluated with CAMAG VideoScan software. Two quantification modes were compared: scanning of whole plate and scanning of manually defined bended tracks. The precision of video-densitometric quantitation of 2D-chromatogram expressed as repeatability of image capturing and track setting was determined. The lowest RSD was obtained when the image was captured at focal distance of 15 mm and F-stop number 2 and the chromatogram quantitatively evaluated using complete scaning with 30 tracks positioned without overlaping.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA