Naslov
Traditional apple cultivars polyphenolic profile analysis and their resistance to infection by Penicillium expansum

Autori
Gotal Skoko, Ana-Marija ; Kovač, Tihomir ; Lončarić, Ante

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XIV Meeting of Young Chemical Engineers / Žižek, Krunoslav ; Katančić, Zvonimir ; Kovačić, Marin - Zagreb, 2022, 39-39

Skup
XIV. susret mladih kemijskih inženjera (SMLKI 2022)

Mjesto i datum
Zagreb, Hrvatska, 24.02.2022. - 25.02.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
apple ; polyphenols ; P. expansum ; patuliln

Sažetak
Traditional apple cultivars contain a higher amount of polyphenolic compounds which are the bearers of apple resistance to plant diseases. Some of the biological activities of polyphenols are antimutagenic, antiphlogistic, antioxidant activity and antifungal activity. Due to that, it seems like traditional apple cultivars possess great potential for apple fruit resistance on Penicillium expansum infection. It is confirmed that traditional apple varieties contain higher levels of polyphenols like flavanols, phenolic acids, procyanidins, dihydrochalcones and flavan- 3-ols. Seven traditional apple cultivars (´Paradija´, ´Srčika´, ´Mašanka´, ´Kanada´, ´Božičnica´, ´Ivandija´ and ´Šampanjka´) from Croatia were analysed by high- performance liquid chromatography with diode-array detectors for the content of polyphenolic compounds. The resistance of traditional apple cultivars to infection by P. expansum (CBS 325.48) was performed mycelia grown on potato dextrose agar at 29 °C ; inoculated 1 cm thick apple slices were incubated at 29 °C until the P. expansum colony reaches the diameter of 9 cm. Patulin content was determined by UPLC-MS/MS method. Results showed that 17 phenolic compounds were detected in traditional apple cultivars. The highest amount of catechin (114.89 ± 2.6 μg/mL), epicatechin (229.81 ± 5.97 μg/mL), chlorogenic acid (3028.78 ± 34.17 μg/mL) and quercetin (18.92 ± 2.38 μg/mL) had ˙Božićnica˙. The highest content of phloridzin (117.75 ± 2.06 μg/mL), phloretin (48.23 ± 0.77 μg/mL), quercetin-3-rutinoside (41.65 ± 0.93 μg/mL) and procyanidin B2 (250.12 ± 2.93 μg/mL) had ˙Srčika˙. Moreover, the highest content of 4-hydrixycinnamic acid (54.59 ± 1.36 μg/mL) and 2-6-dimethoxybenzoic acid (352.8 ± 25.55 μg/mL) had ˙Kanada˙ and the highest content of procyanidin A2 (55.11 ± 1.75 μg/mL) and procyanidin B1 (39.52 ± 1.14 μg/mL) had ˙Paradija˙. However, patulin wasn´t detected in any up mentioned apple cultivars.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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