Naslov
Protein markers in plant development

Autori
Krsnik-Rasol, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3rd Slovenian Symposium on Plant Physiology with international participation - Book of Abstracts / Dolenc Koce, Jasna ; Vodnik, Dominik ; Dermastia, Marina - Ljubljana : Slovenian Society of Plant Physiology, 2002

Skup
3rd Slovenian Symposium on Plant Physiology with international participation

Mjesto i datum
Ljubljana, Slovenija, 25.09.2002. - 27.09.2002

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
protein markers; plant development

Sažetak
After many decades of intensive genome research and impressive revelations on gene structure and function, we are now facing the growing interest in information on proteom, a functional genom complement. Proteom analysis (proteomics) includes the identification and quantification of proteins, understanding of which proteins are present in a particular tissue, which posttranslational modifications occur and how protein activity and interactions are regulated. In the present work, cell differentiation and plant morphogenesis have been studied on in vitro tissue of three model species: sugar beet (Beta vulgaris L.), horseradish (Armoracia lapathifolia Gilib.) and cactus (Mammillaria gracillis Pfeiff.) Normal, habituated and transgenic callus lines and in vitro regenerants were compared with regard to: (1) 1-D and 2-D electrophoretic protein patterns, (2) isoperoxidases and isoesterases activity and composition, (3) pattern of extracellular glycoproteins reacting with the lectin ConA. Developmentally specific protein bands could be established with more accurancy among extracellular protein and isoesterases, than among the cellular protein and isoperoxidases.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA