Naslov
Fluorimetrijsko prepoznavanje DNA i BSA ovisi o povezanosti konjugata triarilboranskog kationa s fluorescentnim supstituentom ili sekvencom peptida
(Fluorimetric Recognition between DNA and BSA Depends on Triarylborone Catione Connectivity to Fluorescent Supstituent or Peptide Sequence)

Autori
Košćak, Marta ; Ban, Željka ; Pintanida, Ivo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Simpozij studenata doktorskih studija PMF-a : knjiga sažetaka = PhD student symposium 2021 : book of abstracts / Barišić, Dajana - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2021, 344-345

ISBN
978-953-6076-90-1

Skup
5. Simpozij studenata doktorskih studija PMF-a = 5th Faculty of Science PhD Student Symposium

Mjesto i datum
Zagreb, Hrvatska, 24.04.2021. - 25.04.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
triarilborani ; DNA/BSA probe ; fluorescencija ; CuAAC click kemija
(triarylboranes ; DNA/BSA probes ; fluorescence ; CuAAC click chemistry)

Sažetak
Small molecule fluorescent probes targeting DNA/RNA and proteins became unavoidable tools for monitoring biological processes due to their biochemical and biomedicinal applications. Until recently boron-containing polycyclic π-systems were considered unstable in water, but in 2012 was shown that planarised triarylboranes can be stable in water and subsequently we showed that such dyes are intriguing fluorescent probes for biomacromolecules. Here, we designed and prepared two novel conjugates of triarylborane cation, differing in the type of “click” attached substituent. Both conjugates showed fluorimetric selectivity between ds-DNA (ct-DNA) and protein (BSA), whereby more flexible conjugate excelled by complete quenching emission for DNA and a strong increase for BSA.

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Kemija

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