Naslov
Interactions of methyl viologens of bis-(4' ethynylpyridyl)arenes with DNA/RNA and their biological activity
(Interactions of methyl viologens of bis-(4'- ethynylpyridyl)arenes with DNA/RNA and their biological activity)

Autori
Košćak, Marta ; Piantanida, Ivo ; Kumar Kole, Goutam ; Marder, Todd B.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27th Croatian Meeting of Chemists and Chemical Engineers and 5th Symposium Vladimir Prelog : Book of Abstracts / Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna - Veli Lošinj : Hrvatsko kemijsko društvo, 2021, 240-240

Skup
27. hrvatski skup kemičara i kemijskih inženjera (27HSKIKI) ; 5. simpozij Vladimir Prelog

Mjesto i datum
Veli Lošinj, Hrvatska, 05.10.2021. - 08.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
viologeni ; DNA/RNA ; cirkularni dikroizam ; MTT test
(viologens ; DNA/RNA ; circular dichroism ; MTT test)

Sažetak
The 4, 4'-bipyridyl salts (viologens), extensively investigated in the past recently again attracted more attention . Based on previous results of Marder et al on bis-(4'-ethynylpyridyl)arenes, here we studied their bis-cationic analogues. 1M and 2M showed only non-selective emission quenching upon binding to various ds-DNA/RNA, at variance to anthracene analogue, 3M, yielding an opposite fluorescence response for GC-DNA (quenching) in comparison to AT-DNA (strong increase). In the CD experiments 3M at ICD bands at λ>300 nm strongly supported intercalation, whereas 2M ICD band (330-450 nm) agreed with DNA/RNA groove binding. MTT assays and confocal microscopy showed that 3M efficiently enters the living cells, accumulating in lysosomes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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