Naslov
Regioselective oxofunctionalization of steroids in deep eutectic solvents

Autori
Radović, Mia ; Cvjetko Bubalo, Marina ; Jurinjak Tušek, Ana ; Radojčić Redovniković, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XIV meeting of young chemical engineers, BOOK OF ABSTRACTS / Žižek, Krunoslav ; Katančić, Zvonimir ; Kovačić, Marin - Zagreb, 2022, 102-102

Skup
XIV. susret mladih kemijskih inženjera (SMLKI 2022)

Mjesto i datum
Zagreb, Hrvatska, 24.02.2022. - 25.02.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
deep eutectic solvents, steroids, oxofunctionalization

Sažetak
Steroid production occupies a prominent place in the pharmaceutical industry, where steroids, after antibiotics, represent the second most important group of pharmaceutics. One of the major transformations in their synthesis path is oxofunctionalization, a regioselective introduction of oxygen into the gonane nucleus that can be catalysed by laccases. With the help of mediator systems, these enzymes can catalyse a wide range of organic substrates. Since most steroid compounds are not water- soluble, the use of deep eutectic solvents (DES) can overcome the disadvantages of common organic solvents. The aim of this research is to investigate the possible use of DES for Trametes versicolor laccase-mediated regioselective oxofunctionalization of 3β- hydroxyandrost-5-en-17-one to 3βhydroxyandrost-5- en-7, 17-dione, an important compound in medicine and pharmaceutics. The Conductor-like Screening Model for Real Solvents (COSMO-RS) was used to predict substrate solubility and to design a two-phase reaction system compatible with all reaction constituents. Three reaction parameters (substrate, mediator and enzyme concentration) were optimised by response surface methodology to obtain a high product yield. This research applies both empirical methods and mathematical models to find one solvent with optimal characteristics for this specific requirement.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA