Naslov
Evaluacija acidobaznog analizatora Radiometer ABL800 FLEX
(Evaluation of acidobase analyser Radiometer ABL800 FLEX)

Autori
Sarić, Ivana ; Šahinović, Ines ; Šerić, Vatroslav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Biochemia Medica 2018 ; 28(Suppl 1):S1-S223 / - Zagreb, 2018, S183-S183

Skup
9. kongres hrvatskog društva za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM)

Mjesto i datum
Zagreb, Hrvatska, 09.05.2018. - 12.05.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Evaluacija, acidobazna ravnoteža, acidobazni analizator
(Evaluation, acidobase homeostasis, acid-base status)

Sažetak
Introduction: Determination of acid-base status is a urgent diagnostic tests that require very short Turnaround- time (TAT) and the determination ability within 24 hours. If the laboratory uses more than one analyzer, it must ensure of their comparability. The aim of this paper is to compare acid-status parameters on the RADIOMETER ABL800FLEX (Radiometer) and RAPIDLAB1265 (Siemens) analyzers. Materials and methods: Two-level commercial controls were used to determine day-to-day accuracy and precision in the ABL800 Flex analyzer. The controls were analyzed in triplicate without time delay and at different times during the day. Evaluation was carried out for 5 days. To test the comparability of the method, 37 samples of arterial blood were sampled in Li-heparin syringe. The pH and pCO2 determination method is potentiometry, pO2 amperometry, while cHCO3 and excess bases (BE) are computational parameters. Precision is presented as a measurement variation coefficient, and the measurement results on two analyzers were compared with the Passing-Bablok regression test. Results: Repeatability and day-to-day precision for pH, CHCO3-, BE, pCO2, pO2 is respectively: < 0.4 %, < 2.6%, < 2.6%, 12% and 20%. Passing-Bablok regression shows the comparability of pCO2, cHCO3 and BE between analyzers. The regression equation for pH is y = 0.3132 (95% CI: 0.0162 to 0.8090) + 0.9549 x (95% CI: 0.8881 to 0.9949). However, the difference between median measurement is not clinically significant (7.41 and 7.39 with RCV = 0.62%). The regression equation for pO2 is y = 0.0426 (95% CI: - 0.1994 to 0.2566) + 0.9542 x (95% CI: 0.9263 to 0.9811). Conclusion: The comparison of two acid-base analyzers is satisfactory for all parameters except pO2. Considering the sensitivity of analyzed samples to air contact, such results are expected and described literally. For the purpose of urgent laboratory diagnostics, the acid-base analyzer ABL800 FLEX demonstrates satisfactory analytical performance and TAT.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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